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The Population Structure of Borrelia lusitaniae Is Reflected by a Population Division of Its Ixodes Vector

2021-04-27Journal article Open access
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dc.contributor.authorNorte, A.C.
dc.contributor.authorBoyer, P.H.
dc.contributor.authorCastillo-Ramirez, S.
dc.contributor.authorChvostac, M.
dc.contributor.authorBrahami, M.O.
dc.contributor.authorRollins, R.E.
dc.contributor.authorWoudenberg, T.
dc.contributor.authorDidyk, Y.M.
dc.contributor.authorDerdakova, M.
dc.contributor.authorNúncio, M.S.
dc.contributor.authorLopes de Carvalho, I.
dc.contributor.authorMargos, G.
dc.contributor.authorFingerle, V.
dc.date.accessioned2022-01-27T17:48:22Z
dc.date.available2022-01-27T17:48:22Z
dc.date.issued2021-04-27
dc.description.abstractPopulations of vector-borne pathogens are shaped by the distribution and movement of vector and reservoir hosts. To study what impact host and vector association have on tick-borne pathogens, we investigated the population structure of Borrelia lusitaniae using multilocus sequence typing (MLST). Novel sequences were acquired from questing ticks collected in multiple North African and European locations and were supplemented by publicly available sequences at the Borrelia Pubmlst database (accessed on 11 February 2020). Population structure of B. lusitaniae was inferred using clustering and network analyses. Maximum likelihood phylogenies for two molecular tick markers (the mitochondrial 16S rRNA locus and a nuclear locus, Tick-receptor of outer surface protein A, trospA) were used to confirm the morphological species identification of collected ticks. Our results confirmed that B. lusitaniae does indeed form two distinguishable populations: one containing mostly European samples and the other mostly Portuguese and North African samples. Of interest, Portuguese samples clustered largely based on being from north (European) or south (North African) of the river Targus. As two different Ixodes species (i.e., I. ricinus and I. inopinatus) may vector Borrelia in these regions, reference samples were included for I. inopinatus but did not form monophyletic clades in either tree, suggesting some misidentification. Even so, the trospA phylogeny showed a monophyletic clade containing tick samples from Northern Africa and Portugal southoftheriverTagussuggestingapopulationdivisioninIxodesonthislocus. Thepatternmirrored the clustering of B. lusitaniae samples, suggesting a potential co-evolution between tick and Borrelia populations that deserve further investigation.pt_PT
dc.description.sponsorshipThis research was financially supported by the Slovak Research and Development Agency (grant number APVV-16-0463), by the Fundação para a Ciência e a Tecnologia by the transitory norm contract DL57/2016/CP1370/CT89 to Ana Cláudia Norte and MARE (MARE-UID/MAR/04292/2020), and by the National Institute of Health Doutor Ricardo Jorge, Lisbon, Portugal. The National Reference Center for Borrelia was supported by the Robert-Koch Institute, Berlin, Germanypt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMicroorganisms. 2021 Apr 27;9(5):933. doi: 10.3390/microorganisms9050933pt_PT
dc.identifier.doi10.3390/microorganisms9050933pt_PT
dc.identifier.issn2076-2607
dc.identifier.urihttp://hdl.handle.net/10400.18/7885
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationMARE-UID/MAR/04292/2020pt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2076-2607/9/5/933pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectPopulation Structurept_PT
dc.subjectVector Associationpt_PT
dc.subjectBorrelia lusitaniaept_PT
dc.subjectIxodespt_PT
dc.subjectMLSTpt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.titleThe Population Structure of Borrelia lusitaniae Is Reflected by a Population Division of Its Ixodes Vectorpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage933pt_PT
oaire.citation.issue5pt_PT
oaire.citation.startPage933pt_PT
oaire.citation.titleMicroorganismspt_PT
oaire.citation.volume9pt_PT
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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