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The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders

2021-02-06Journal article Open access
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dc.contributor.authorMilić, Mirta
dc.contributor.authorCeppi, Marcello
dc.contributor.authorBruzzone, Marco
dc.contributor.authorAzqueta, Amaya
dc.contributor.authorBrunborg, Gunnar
dc.contributor.authorGodschalk, Roger
dc.contributor.authorKoppen, Gudrun
dc.contributor.authorLangie, Sabine
dc.contributor.authorMøller, Peter
dc.contributor.authorTeixeira, João Paulo
dc.contributor.authorAlija, Avdulla
dc.contributor.authorAnderson, Diana
dc.contributor.authorAndrade, Vanessa
dc.contributor.authorAndreoli, Cristina
dc.contributor.authorAsllani, Fisnik
dc.contributor.authorBangkoglu, Ezgi Eyluel
dc.contributor.authorBarančoková, Magdalena
dc.contributor.authorBasaran, Nursen
dc.contributor.authorBoutet-Robinet, Elisa
dc.contributor.authorBuschini, Annamaria
dc.contributor.authorCavallo, Delia
dc.contributor.authorCosta Pereira, Cristiana
dc.contributor.authorCosta, Carla
dc.contributor.authorCosta, Solange
dc.contributor.authorDa Silva, Juliana
dc.contributor.authorDel Boˊ, Cristian
dc.contributor.authorDimitrijević Srećković, Vesna
dc.contributor.authorDjelić, Ninoslav
dc.contributor.authorDobrzyńska, Malgorzata
dc.contributor.authorDuračková, Zdenka
dc.contributor.authorDvořáková, Monika
dc.contributor.authorGajski, Goran
dc.contributor.authorGalati, Serena
dc.contributor.authorGarcía Lima, Omar
dc.contributor.authorGiovannelli, Lisa
dc.contributor.authorGoroshinskaya, Irina A.
dc.contributor.authorGrindel, Annemarie
dc.contributor.authorGutzkow, Kristine B.
dc.contributor.authorHernández, Alba
dc.contributor.authorHernández, Carlos
dc.contributor.authorHolven, Kirsten B.
dc.contributor.authorIbero-Baraibar, Idoia
dc.contributor.authorOttestad, Inger
dc.contributor.authorKadioglu, Ela
dc.contributor.authorKažimirová, Alena
dc.contributor.authorKuznetsova, Elena
dc.contributor.authorLadeira, Carina
dc.contributor.authorLaffon, Blanca
dc.contributor.authorLamonaca, Palma
dc.contributor.authorLebailly, Pierre
dc.contributor.authorLouro, Henriqueta
dc.contributor.authorMandina Cardoso, Tania
dc.contributor.authorMarcon, Francesca
dc.contributor.authorMarcos, Ricard
dc.contributor.authorMoretti, Massimo
dc.contributor.authorMoretti, Silvia
dc.contributor.authorNajafzadeh, Mojgan
dc.contributor.authorNemeth, Zsuzsanna
dc.contributor.authorNeri, Monica
dc.contributor.authorNovotna, Bozena
dc.contributor.authorOrlow, Irene
dc.contributor.authorPaduchova, Zuzana
dc.contributor.authorPastor, Susana
dc.contributor.authorPerdry, Hervé
dc.contributor.authorSpremo-Potparević, Biljana
dc.contributor.authorRamadhani, Dwi
dc.contributor.authorRiso, Patrizia
dc.contributor.authorRohr, Paula
dc.contributor.authorRojas, Emilio
dc.contributor.authorRossner, Pavel
dc.contributor.authorSafar, Anna
dc.contributor.authorSardas, Semra
dc.contributor.authorSilva, Maria João
dc.contributor.authorSirota, Nikolay
dc.contributor.authorSmolkova, Bozena
dc.contributor.authorStaruchova, Marta
dc.contributor.authorStetina, Rudolf
dc.contributor.authorStopper, Helga
dc.contributor.authorSurikova, Ekaterina I.
dc.contributor.authorUlven, Stine M.
dc.contributor.authorUrsini, Cinzia Lucia
dc.contributor.authorValdiglesias, Vanessa
dc.contributor.authorValverde, Mahara
dc.contributor.authorVodicka, Pavel
dc.contributor.authorVolkovova, Katarina
dc.contributor.authorWagner, Karl-Heinz
dc.contributor.authorŽivković, Lada
dc.contributor.authorDušinská, Maria
dc.contributor.authorCollins, Andrew R.
dc.contributor.authorBonassi, Stefano
dc.date.accessioned2022-01-10T15:32:27Z
dc.date.available2022-01-10T15:32:27Z
dc.date.issued2021-02-06
dc.descriptionReview
dc.description.abstractThe alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent a valuable tool to meet this challenge. The aims of hCOMET were to establish reference values for the level of DNA damage in humans, to investigate the effect of host factors, lifestyle and exposure to genotoxic agents, and to compare different sources of assay variability. A database of 19,320 subjects was generated, pooling data from 105 studies run by 44 laboratories in 26 countries between 1999 and 2019. A mixed random effect log-linear model, in parallel with a classic meta-analysis, was applied to take into account the extensive heterogeneity of data, due to descriptor, specimen and protocol variability. As a result of this analysis interquartile intervals of DNA strand breaks (which includes alkali-labile sites) were reported for tail intensity, tail length, and tail moment (comet assay descriptors). A small variation by age was reported in some datasets, suggesting higher DNA damage in oldest age-classes, while no effect could be shown for sex or smoking habit, although the lack of data on heavy smokers has still to be considered. Finally, highly significant differences in DNA damage were found for most exposures investigated in specific studies. In conclusion, these data, which confirm that DNA damage measured by the comet assay is an excellent biomarker of exposure in several conditions, may contribute to improving the quality of study design and to the standardization of results of the comet assay in human populations.pt_PT
dc.description.abstractHighlights: The alkaline comet assay is a popular methods for assessing DNA damage in humans; A database of 19,320 subjects from 44 laboratories in 26 countries was analysed; A range of measures estimating baseline DNA damage is provided for %T, TL, and TM; None or limited effect was found for sex, age, and major confounding factors; The comet assay efficiently detected specific genotoxic exposures.pt_PT
dc.description.sponsorshipCOST Action hCOMET CA15132, supported by COST (European Cooperation in Science and Technology www.cost.eu)pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMutat Res Rev Mutat Res. Jan-Jun 2021;787:108371. doi: 10.1016/j.mrrev.2021.108371. Epub 2021 Feb 6. Reviewpt_PT
dc.identifier.doi10.1016/j.mrrev.2021.108371pt_PT
dc.identifier.issn1383-5742
dc.identifier.urihttp://hdl.handle.net/10400.18/7838
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S1383574221000089?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectEnvironmental Genotoxicitypt_PT
dc.subjectDNA Damagept_PT
dc.subjectComet Assaypt_PT
dc.subjectHuman Biomonitoringpt_PT
dc.subjectPooled Analysispt_PT
dc.subjectBiomarkerspt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.subjectGenómica Funcional e Estruturalpt_PT
dc.titleThe hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounderspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage108371pt_PT
oaire.citation.titleMutation Research/Reviews in Mutation Researchpt_PT
oaire.citation.volume787pt_PT
person.familyNameLouro
person.familyNameSilva
person.givenNameHenriqueta
person.givenNameMaria Joao
person.identifier157627
person.identifier.ciencia-id721D-2BB1-7DB1
person.identifier.ciencia-id7710-643D-97A3
person.identifier.orcid0000-0001-9744-7332
person.identifier.orcid0000-0002-6060-0716
person.identifier.scopus-author-id6507971479
person.identifier.scopus-author-id55944437600
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication2361a951-8b9a-4b90-92d6-f6384003a242
relation.isAuthorOfPublicationa7763685-7c34-468d-b958-9dd0aca66db4
relation.isAuthorOfPublication.latestForDiscovery2361a951-8b9a-4b90-92d6-f6384003a242

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