Critical Aspects for Detection of Coxiella burnetii

Mori, M.; Mertens, K.; Cutler, S.J.; Santos, A.S.

Publication

Critical Aspects for Detection of Coxiella burnetii

2017-01Journal article

dc.contributor.author	Mori, M.
dc.contributor.author	Mertens, K.
dc.contributor.author	Cutler, S.J.
dc.contributor.author	Santos, A.S.
dc.date.accessioned	2018-02-01T15:32:26Z
dc.date.available	2018-02-01T15:32:26Z
dc.date.issued	2017-01
dc.description.abstract	Coxiella burnetii is a globally distributed zoonotic γ-proteobacterium with an obligatory intracellular lifestyle. It is the causative agent of Q fever in humans and of coxiellosis among ruminants, although the agent is also detected in ticks, birds, and various other mammalian species. Requirements for intracellular multiplication together with the necessity for biosafety level 3 facilities restrict the cultivation of C. burnetii to specialized laboratories. Development of a novel medium formulation enabling axenic growth of C. burnetii has facilitated fundamental genetic studies. This review provides critical insights into direct diagnostic methods currently available for C. burnetii. It encompasses molecular detection methods, isolation, and propagation of the bacteria and its genetic characterization. Differentiation of C. burnetii from Coxiella-like organisms is an essential diagnostic prerequisite, particularly when handling and analyzing ticks.	pt_PT
dc.description.version	info:eu-repo/semantics/publishedVersion	pt_PT
dc.identifier.citation	Vector Borne Zoonotic Dis. 2017 Jan;17(1):33-41. doi: 10.1089/vbz.2016.1958.	pt_PT
dc.identifier.doi	10.1089/vbz.2016.1958	pt_PT
dc.identifier.issn	1530-3667
dc.identifier.uri	http://hdl.handle.net/10400.18/4901
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.publisher	Mary Ann Liebert/Society for Zoonotic Ecology and Epidemiology (SocZEE)	pt_PT
dc.relation.publisherversion	https://doi.org/10.1089/vbz.2016.1958	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by-nc/4.0/	pt_PT
dc.subject	Infecções Sistémicas e Zoonoses	pt_PT
dc.subject	Coxiella burnetii	pt_PT
dc.subject	PCR	pt_PT
dc.subject	Axenic Culture	pt_PT
dc.subject	Direct Diagnosis	pt_PT
dc.subject	Genome Sequencing	pt_PT
dc.subject	Isolation	pt_PT
dc.title	Critical Aspects for Detection of Coxiella burnetii	pt_PT
dc.type	journal article
dspace.entity.type	Publication
oaire.citation.endPage	41	pt_PT
oaire.citation.issue	1	pt_PT
oaire.citation.startPage	33	pt_PT
oaire.citation.title	Vector-Borne and Zoonotic Diseases	pt_PT
oaire.citation.volume	17	pt_PT
rcaap.rights	embargoedAccess	pt_PT
rcaap.type	article	pt_PT