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Toxicological assessment of silica-coated iron oxide nanoparticles in human astrocytes

2018-08Artigo científico Acesso restrito
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dc.contributor.authorFernández-Bertólez, Natalia
dc.contributor.authorCosta, Carla
dc.contributor.authorBrandão, Fátima
dc.contributor.authorKiliç, Gözde
dc.contributor.authorDuarte, José Alberto
dc.contributor.authorTeixeira, Joao Paulo
dc.contributor.authorPásaro, Eduardo
dc.contributor.authorValdiglesias, Vanessa
dc.contributor.authorLaffon, Blanca
dc.date.accessioned2019-03-25T17:17:58Z
dc.date.available2019-03-25T17:17:58Z
dc.date.issued2018-08
dc.description.abstractIron oxide nanoparticles (ION) have great potential for an increasing number of medical and biological applications, particularly those focused on nervous system. Although ION seem to be biocompatible and present low toxicity, it is imperative to unveil the potential risk for the nervous system associated to their exposure, especially because current data on ION effects on human nervous cells are scarce. Thus, in the present study potential toxicity associated with silica-coated ION (S-ION) exposure was evaluated on human A172 glioblastoma cells. To this aim, a complete toxicological screening testing several exposure times (3 and 24 h), nanoparticle concentrations (5-100 μg/ml), and culture media (complete and serum-free) was performed to firstly assess S-ION effects at different levels, including cytotoxicity - lactate dehydrogenase assay, analysis of cell cycle and cell death production - and genotoxicity - H2AX phosphorylation assessment, comet assay, micronucleus test and DNA repair competence assay. Results obtained showed that S-ION exhibit certain cytotoxicity, especially in serum-free medium, related to cell cycle disruption and cell death induction. However, scarce genotoxic effects and no alteration of the DNA repair process were observed. Results obtained in this work contribute to increase the knowledge on the impact of ION on the human nervous system cells.pt_PT
dc.description.sponsorshipThis work was funded by Xunta de Galicia (ED431B 2016/013). V. Valdiglesias was supported by a Xunta de Galicia postdoctoral fellowship (reference ED481B 2016/190-0). N. Fernández-Bertólez was supported by an INDITEX-UDC fellowship. F. Brandão is supported by the grant SFRH/BD/101060/2014, funded by FCT (financing subsided by national fund of MCTES). Authors would also like to acknowledge COST Action CA15132 “The comet assay as a human biomonitoring tool (hCOMET)”pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFood Chem Toxicol. 2018 Aug;118:13-23. doi: 10.1016/j.fct.2018.04.058. Epub 2018 Apr 27pt_PT
dc.identifier.doi10.1016/j.fct.2018.04.058pt_PT
dc.identifier.issn0278-6915
dc.identifier.urihttp://hdl.handle.net/10400.18/6304
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier/ Pergamonpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0278691518302813?via%3Dihubpt_PT
dc.subjectAstrocytespt_PT
dc.subjectCarcinogenicity Testspt_PT
dc.subjectCell Cyclept_PT
dc.subjectCell Deathpt_PT
dc.subjectCell Line, Tumorpt_PT
dc.subjectCulture Mediapt_PT
dc.subjectDNA Repairpt_PT
dc.subjectFerric Compoundspt_PT
dc.subjectHistonespt_PT
dc.subjectHumanspt_PT
dc.subjectMetal Nanoparticlespt_PT
dc.subjectMicroscopy, Electron, Transmissionpt_PT
dc.subjectMutagenicity Testspt_PT
dc.subjectNervous Systempt_PT
dc.subjectPhosphorylationpt_PT
dc.subjectSilicon Dioxidept_PT
dc.subjectCoated Materials, Biocompatiblept_PT
dc.subjectIron Oxide Nanoparticlespt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleToxicological assessment of silica-coated iron oxide nanoparticles in human astrocytespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage23pt_PT
oaire.citation.startPage13pt_PT
oaire.citation.titleFood and Chemical Toxicologypt_PT
oaire.citation.volume118pt_PT
rcaap.embargofctPolítica editorial da revistapt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

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