Publication
IncX4 plasmid carrying the new mcr-1.9 gene variant in a CTX-M-8-producing Escherichia coli isolate recovered from swine
| dc.contributor.author | Manageiro, Vera | |
| dc.contributor.author | Clemente, Lurdes | |
| dc.contributor.author | Romão, Raquel | |
| dc.contributor.author | Silva, Catarina | |
| dc.contributor.author | Vieira, Luís | |
| dc.contributor.author | Ferreira, Eugénia | |
| dc.contributor.author | Caniça, Manuela | |
| dc.date.accessioned | 2020-05-03T17:48:40Z | |
| dc.date.available | 2020-05-03T17:48:40Z | |
| dc.date.issued | 2019-03-14 | |
| dc.description.abstract | We studied a commensal colistin-resistant Escherichia coli isolated from a swine cecum sample collected at a slaughter, in Portugal. Antimicrobial susceptibility phenotype of E. coli LV23529 showed resistance to colistin at a minimum inhibitory concentration of 4 mg/L. Whole genome of E. coli LV23529 was sequenced using a MiSeq system and the assembled contigs were analyzed for the presence of antibiotic resistance and plasmid replicon types using bioinformatics tools. We report a novel mcr-1 gene variant (mcr-1.9), carried by an IncX4 plasmid, where one-point mutation at nucleotide T1238C leads to Val413Ala substitution. The mcr-1.9 genetic context was characterized by an IS26 element upstream of the mcr-pap2 element and by the absence of ISApl1. Bioinformatic analysis also revealed genes conferring resistance to β-lactams, sulphamethoxazole, trimethoprim, chloramphenicol and colistin, corresponding to the phenotype noticed. Moreover, we highlight the presence of mcr-1.9 plus blaCTX-M-8, a blaESBL gene rarely detected in Europe in isolates of animal origin; these two genes were located on different plasmids with 33,303 and 89,458 bp, respectively. MCR-1.9-harboring plasmid showed high identity to other X4-type mcr-1-harboring plasmids characterized worldwide, which strongly suggests that the presence of PMCR-encoding genes in food-producing animals, such as MCR-1.9, represent a potential threat to humans, as it is located in mobile genetic elements that have the potential to spread horizontally. | pt_PT |
| dc.description.sponsorship | VM was supported by Fundação para a Ciência e a Tecnologia (FCT) fellowship (Grant No. SFRH/BPD/77486/2011), financed by the European Social Funds (COMPETE-FEDER), and National Funds of the Portuguese Ministry of Education and Science (POPH-QREN). The authors thank to FCT for the project grant UID/MULTI/00211/2013. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Front Microbiol. 2019 Mar 14;10:367. doi: 10.3389/fmicb.2019.00367. eCollection 2019 | pt_PT |
| dc.identifier.doi | 10.3389/fmicb.2019.00367 | pt_PT |
| dc.identifier.issn | 1664-302X | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/6585 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Frontiers Media | pt_PT |
| dc.relation | EVOLUTIONARY POTENTIAL OF OXYIMINO-BETA-LACTAM RESISTANCE DETERMINANTS IN ANIMALS AND IDENTIFICATION OF NEW ENZYME-INHIBITORS FOR VETERINARY USE | |
| dc.relation.publisherversion | https://www.frontiersin.org/articles/10.3389/fmicb.2019.00367/full | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | pt_PT |
| dc.subject | MCR-1.9 | pt_PT |
| dc.subject | Plasmid-mediated Colistin Resistance | pt_PT |
| dc.subject | IncX4 | pt_PT |
| dc.subject | CTX-M-8 | pt_PT |
| dc.subject | Escherichia coli | pt_PT |
| dc.subject | Portugal | pt_PT |
| dc.subject | Resistência aos Antimicrobianos | pt_PT |
| dc.title | IncX4 plasmid carrying the new mcr-1.9 gene variant in a CTX-M-8-producing Escherichia coli isolate recovered from swine | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | EVOLUTIONARY POTENTIAL OF OXYIMINO-BETA-LACTAM RESISTANCE DETERMINANTS IN ANIMALS AND IDENTIFICATION OF NEW ENZYME-INHIBITORS FOR VETERINARY USE | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F77486%2F2011/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F00211%2F2013/PT | |
| oaire.citation.startPage | 367 | pt_PT |
| oaire.citation.title | Frontiers in Microbiology | pt_PT |
| oaire.citation.volume | 10 | pt_PT |
| oaire.fundingStream | 5876 | |
| person.familyName | Manageiro | |
| person.givenName | Vera | |
| person.identifier | 223943 | |
| person.identifier.ciencia-id | 361B-9EDA-DEE7 | |
| person.identifier.orcid | 0000-0001-7729-4199 | |
| person.identifier.rid | M-9226-2013 | |
| person.identifier.scopus-author-id | 16480778900 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
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