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Evaluation of FASP for Mass Spectrometry-Based Untargeted Metabolomics analysis of urine samples

2026-07-03Artigo científico Acesso aberto
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datacite.subject.fosCiências Naturais::Ciências Biológicas
dc.contributor.authorMousa, Muath Khairi
dc.contributor.authorCarvalho, Luis B.
dc.contributor.authorGiddey, Alexander D.
dc.contributor.authorFigueiredo, André
dc.contributor.authorAl-Hroub, Hamza
dc.contributor.authorSemreen, Mohammad H.
dc.contributor.authorUddin, Mohammed
dc.contributor.authorSantos, Hugo M.
dc.contributor.authorSoares, Nelson C.
dc.date.accessioned2026-10-01T14:18:19Z
dc.date.available2026-10-01T14:18:19Z
dc.date.issued2026-07-03
dc.description(Special Issue: Published as part of Journal of Proteome Research special issue “Multiomics in Africa and the Middle East)
dc.description.abstractFilter-Aided Sample Preparation (FASP) is a well-established method in proteomics, yet its potential for the parallel recovery of metabolites remains largely unexplored. Herein, we evaluate the performance of FASP as a straightforward workflow for the simultaneous isolation of protein and corresponding metabolite fractions from a single urine sample. The FASP-based LC-MS/MS approach for both proteomics and metabolomics analysis identified 3,163 nonredundant peptides corresponding to 957 unique protein groups. The metabolomic profile comparison of three urine fractions, specifically FASP-concentrated, FASP flow-through, and raw samples, resulted in the identification of 176 common metabolites. Next, as a proof-of-concept, the FASP protocol was applied to compare the metabolomic profiles of clinical urine samples from healthy individuals (n = 13) and patients with Ta bladder cancer (n = 12). The metabolomic modulation was consistent with previously reported findings, highlighting perturbations in phenylacetate, purine, and tryptophan metabolism, as reflected by changes in metabolites such as adenosine monophosphate (AMP), phenylacetic acid, glutamine, cytosine, and l-tryptophan. FASP protocol can be effectively adapted for the concurrent profiling of both proteomic and metabolomic fractions from urine samples. Thus, FASP-based workflow represents a viable alternative for single-step sample preparation, facilitating subsequent quantitative multiomics data integration.eng
dc.description.sponsorshipStudy was supported by the Center for Applied and Translational Genomics (CATG) at Mohammed Bin Rashid University of Medicine and Health Sciences (MBRU), Dubai, United Arab Emirates, and by Dubai Health. Internal grant MBRU, Dubai Health, College of Medicine grants cycle 2025/2027. Grant Reference No.: MRBU-CM-RG2025-14, title: Development of multiomics biomarker test to predict the risk of premature myocardial infarction (MI) in the UAE. The University of Sharjah for financial support. This study was partially financially supported by the University of Sharjah Targeted grant No.24011101105 (M.H.S.).))
dc.identifier.citationJ Proteome Res. 2026 Jul 3;25(7):3769-3777. doi: 10.1021/acs.jproteome.6c00173. Epub 2026 Jun 18
dc.identifier.doi10.1021/acs.jproteome.6c00173
dc.identifier.eissn1535-3907
dc.identifier.issn1535-3893
dc.identifier.pmid42314122
dc.identifier.urihttp://hdl.handle.net/10400.18/11367
dc.language.isoeng
dc.peerreviewedyes
dc.publisherAmerican Chemical Society
dc.relation.hasversionhttps://pubs.acs.org/jprobs/article/25/7/3769/5162800/Evaluation-of-FASP-for-Mass-Spectrometry-Based
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectBladder Cancer
dc.subjectFASP
dc.subjectMetabolomics
dc.subjectMultiomics
dc.subjectProteomics
dc.subjectSample Preparation
dc.subjectUrine
dc.subjectSample Preparation
dc.subjectGenómica Funcional e Estrutural
dc.titleEvaluation of FASP for Mass Spectrometry-Based Untargeted Metabolomics analysis of urine sampleseng
dc.typejournal article
dcterms.referenceshttps://pubs.acs.org/jprobs/article/25/7/3769/5162800/Evaluation-of-FASP-for-Mass-Spectrometry-Based#supplementary-data
dspace.entity.typePublication
oaire.citation.endPage3777
oaire.citation.issue7
oaire.citation.startPage3769
oaire.citation.titleJournal of Proteome Research
oaire.citation.volume25
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85

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