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Expression of miR146-a, an inflammation-associated microRNA, in Mesial Temporal Lobe Epilepsy

2015-05-20Conference object Open access
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dc.contributor.authorLeal, B.
dc.contributor.authorCarvalho, C.
dc.contributor.authorChaves, J.
dc.contributor.authorBettencourt, A.
dc.contributor.authorFreitas, J.
dc.contributor.authorLopes, J.
dc.contributor.authorRamalheira, J.
dc.contributor.authorMartins da Silva, A.
dc.contributor.authorCosta, P. P.
dc.contributor.authorMartins da Silva, B.
dc.date.accessioned2021-04-08T14:59:10Z
dc.date.available2021-04-08T14:59:10Z
dc.date.issued2015-05-20
dc.descriptionOral Sessions publicado em: European Journal of Neurology. 2015;22(Suppl.1):21. doi: 10.1111/ene.12805. Disponível em: https://onlinelibrary.wiley.com/doi/abs/10.1111/ene.12805pt_PT
dc.description.abstractBackground: Neuroinflammation appears as an important epileptogenic mechanism. MicroRNAs (miRNA) are small non-coding RNA molecules that function as post-transcriptional regulators of gene expression. MicroRNas control different biological process including immune system homeostasis and function. Several evidences, both in patients and animal studies, have demonstrated an abnormal brain expression of miR-146a in Mesial Temporal Lobe Epilepsy. Knowing that miR expression is very stable in biological fluids such as plasma or serum our aim was to characterize miR146a expression in serum of MTLE patients. Methods: Expression levels of miR146a and U6B small nuclear RNA gene (reference gene) were quantified by Real-Time PCR in serum of 14 MTLE patients all with Hippocampal Sclerosis (6F, 8M, mean age= 44.1±11.7 years, age of onset= 13.5±10.6 years, 7 with Febrile Seizures antecedents). A group of 10 healthy individuals was used as control. Relative expression values were calculated using the 2-ΔΔCt method. Results: We observed that expression of miR146a was 2 fold higher in MTLE-HS patients than in controls. Conclusion: The results obtained in serum are in accordance with the results obtained from brain tissue of epileptic patients. This may confirm that miR-146a is a suitable biomarker of epileptogenesis. Additionally, it is thought that miR-146a has a role in fine-tuning the response to cytokines during epileptogenesis. Nevertheless its importance in epilepsy development it is yet not fully understood. The comprehension of this role may be relevant for the development of new therapeutic strategies.pt_PT
dc.description.sponsorshipBICE Tecnifar Grant 2014pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/7673
dc.language.isoengpt_PT
dc.peerreviewednopt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectResearch Subject Categories::MEDICINE::Microbiology, immunology, infectious diseases::Immunology::Immunobiologypt_PT
dc.subjectMesial Temporal Lobe Epilepsypt_PT
dc.subjectInflammationpt_PT
dc.subjectEpigeneticspt_PT
dc.subjectmiRNAspt_PT
dc.subjectDeterminantes da Saúde e da Doençapt_PT
dc.subjectDoenças Genéticaspt_PT
dc.titleExpression of miR146-a, an inflammation-associated microRNA, in Mesial Temporal Lobe Epilepsypt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceBerlin, Germanypt_PT
oaire.citation.title1st Congress of the European Academy of Neurology, 20-23 June 2015pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT

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