Repository logo
 
Publication

Experimental supporting data on DIS3L2 over nonsense-mediated mRNA decay targets in human cells

2019-12-06Journal article Open access
Simple item page
dc.contributor.authorda Costa, Paulo J.
dc.contributor.authorMenezes, Juliane
dc.contributor.authorSaramago, Margarida
dc.contributor.authorGarcía-Moreno, Juan F.
dc.contributor.authorSantos, Hugo A.
dc.contributor.authorGama-Carvalho, Margarida
dc.contributor.authorArraiano, Cecília M.
dc.contributor.authorViegas, Sandra C.
dc.contributor.authorRomão, Luísa
dc.date.accessioned2020-04-23T18:11:13Z
dc.date.available2020-04-23T18:11:13Z
dc.date.issued2019-12-06
dc.description.abstractIn this article, we present supportive data related to the research article “A role for DIS3L2 over natural nonsense-mediated mRNA decay targets in human cells” [1], where interpretation of the data presented here is available. Indeed, here we analyze the impact of the DIS3L2 exoribonuclease over nonsense-mediated mRNA decay (NMD)-targets. Specifically, we present data on: a) the expression of various reporter human β-globin mRNAs, monitored by Northern blot and RT-qPCR, before and after altering DIS3L2 levels in HeLa cells, and b) the gene expression levels of deregulated transcripts generated by re-analyzing publicly available data from UPF1-depleted HeLa cells that were further cross-referenced with a dataset of transcripts upregulated in DIS3L2-depleted cells. These analyses revealed that DIS3L2 regulates the levels of a subset of NMD-targets. These data can be valuable for researchers interested in the NMD mechanism.pt_PT
dc.description.sponsorshipThis work was partially supported by Fundação para a Ciencia e a Tecnologia (FCT) (PTFC/BIM-MEC/3749/2014 to LR and UID/MULTI/04046/2013 to BioISI). PJdC, HAS and JFG-M are recipients of a fellowship from BioSys PhD programme (SFRH/BD/52495/2014, SFRH/BD/52492/2014, and PD/BD/ 142898/2018, respectively) and JM is a postdoctoral fellow (SFRH/BPD/98360/2013) from FCT. Work at ITQB-NOVA was financially supported by: Project LISBOA-01-0145-FEDER-007660 funded by the European Regional Development Fund (FEDER) through COMPETE2020 - Programa Operacional Competitividade e Internacionalização (POCI) and by FCT funds: PTDC/BIA-MIC/1399/2014 to CMA and PTFC/BIM-MEC/3749/2014 to SCV. SCV was financed by program IF of FCT (IF/00217/2015). MS was financed by an FCT contract according to DL57/2016 [SFRH/BPD/109464/2015]pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationData Brief . 2019 Dec 6;28:104943. doi: 10.1016/j.dib.2019.104943. eCollection 2020 Febpt_PT
dc.identifier.doi10.1016/j.dib.2019.104943pt_PT
dc.identifier.issn2352-3409
dc.identifier.urihttp://hdl.handle.net/10400.18/6501
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S2352340919312983?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nd/4.0/pt_PT
dc.subjectDIS3L2pt_PT
dc.subjectNMDpt_PT
dc.subjectNMD-targetspt_PT
dc.subjectUPF1pt_PT
dc.subjectmRNA Degradationpt_PT
dc.subjectmRNA Surveillancept_PT
dc.subjectDoenças Genéticaspt_PT
dc.subjectGenómica Funcional e Estruturalpt_PT
dc.titleExperimental supporting data on DIS3L2 over nonsense-mediated mRNA decay targets in human cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04046%2F2013/PT
oaire.citation.startPage104943pt_PT
oaire.citation.titleData in Briefpt_PT
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctDe acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationdc84f768-e6f2-4eea-b294-6c8ebbd1a156
relation.isProjectOfPublication.latestForDiscoverydc84f768-e6f2-4eea-b294-6c8ebbd1a156

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
da Costa P et al. 2019.pdf
Size:
500.72 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

DGH - Artigos em revistas internacionais