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Comparative assessment of the acute toxicity of commonly used metal nanoparticles in two in vitro models of human barriers

2021-05-05Conference object Open access
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dc.contributor.authorPires, J.
dc.contributor.authorMoreira, L.
dc.contributor.authorTeixeira, João
dc.contributor.authorFraga, Sónia
dc.date.accessioned2022-07-10T19:57:47Z
dc.date.available2022-07-10T19:57:47Z
dc.date.issued2021-05-05
dc.description.abstractMetal nanoparticles (M-NP) have application in several areas such as industry, environment, agriculture, and biomedicine. Consequently, human exposure to these nanosized materials is increasing, which raises serious concerns regarding their safety to the human health and the environment. Biological barriers are important lines of defence to xenobiotics, thus expected targets for M-NP. The present study investigated the in vitro toxicity of different types of M-NP in two cell models of biological barriers: human intestinal (Caco-2) and trophoblastic (BeWo clone b30) epithelial cells. Cells were exposed for 24 h to varied concentrations (0.8-48 µg/cm2) of M NP of different chemical composition (Au, Ag, TiO2), primary size (10, 30 and 60 nm), capping (citrate, PEG) and crystal structure (rutile, anatase) and toxicity assessed by determining changes in cell morphology, metabolic activity, plasma membrane integrity, generation of intracellular reactive oxygen species (ROS) and intracellular ATP levels. Our data show that the potential toxicity of the tested M-NPs is similar for both cell lines with AgNPs > AuNPs > TiO2NPs, being the effects more visible at higher concentrations. The influence of the size in the cytotoxic-induced effects was more evident for AgNP than for AuNP, with the smaller NP causing more toxicity, being the BeWo cells more sensitive to these M-NP. In addition, PEG-capping effectively attenuated AuNP-induced toxicity both in Caco-2 and BeWo cells. Only cells exposed to AgNP exhibited significant increased levels of ROS. Thus, our data support that the physicochemical properties of the nanomaterials, in this particular case of M-NP, is an important determinant of their cytotoxicity and that intestinal and trophoblastic cells exhibit different sensitivity to the tested M-NP. Future studies would be useful to further explore the effects of M-NP in the human barrierspt_PT
dc.description.sponsorshipNanoBioBarriers (PTDC/MED-TOX/31162/2017)pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/8222
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectMetal Nanoparticlespt_PT
dc.subjectGenotoxicitypt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleComparative assessment of the acute toxicity of commonly used metal nanoparticles in two in vitro models of human barrierspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FMED-TOX%2F31162%2F2017/PT
oaire.citation.conferencePlacePorto, Portugal (online)pt_PT
oaire.citation.title14ª edição do Encontro de Investigação Jovem da U.Porto, 5-7 maio 2021pt_PT
oaire.fundingStream9471 - RIDTI
person.familyNameVaz Moreira
person.familyNamePaulo Teixeira
person.familyNameFraga
person.givenNameLuciana Maria
person.givenNameJoao
person.givenNameSónia
person.identifierAAD-8155-2020
person.identifier230313
person.identifierD-8155-2013
person.identifier.ciencia-idF81D-105A-1500
person.identifier.ciencia-idDC1F-3ED2-2707
person.identifier.ciencia-id0717-20FD-1212
person.identifier.orcid0000-0001-5369-5422
person.identifier.orcid0000-0001-8693-5250
person.identifier.orcid0000-0001-9386-2336
person.identifier.ridF-5445-2013
person.identifier.scopus-author-id36097134700
person.identifier.scopus-author-id7005283366
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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