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Unveiling the Toxicity of Fine and Nano-Sized Airborne Particles Generated from Industrial Thermal Spraying Processes in Human Alveolar Epithelial Cells

2022-04-13Journal article Open access
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dc.contributor.authorBessa, Maria João
dc.contributor.authorBrandão, Fátima
dc.contributor.authorFokkens, Paul H.B.
dc.contributor.authorLeseman, Daan L.A.C.
dc.contributor.authorBoere, A. John F.
dc.contributor.authorCassee, Flemming R.
dc.contributor.authorSalmatonidis, Apostolos
dc.contributor.authorViana, Mar
dc.contributor.authorMonfort, Eliseo
dc.contributor.authorFraga, Sónia
dc.contributor.authorTeixeira, João Paulo
dc.date.accessioned2023-01-25T10:58:31Z
dc.date.available2023-01-25T10:58:31Z
dc.date.issued2022-04-13
dc.descriptionThis article belongs to the Special Issue Toxicity of Nanomaterials and Legacy Contaminants: Risks to the Environment and Human Health.pt_PT
dc.description.abstractHigh-energy industrial processes have been associated with particle release into workplace air that can adversely affect workers’ health. The present study assessed the toxicity of incidental fine (PGFP) and nanoparticles (PGNP) emitted from atmospheric plasma (APS) and high-velocity oxy-fuel (HVOF) thermal spraying. Lactate dehydrogenase (LDH) release, 2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate (WST-1) metabolisation, intracellular reactive oxygen species (ROS) levels, cell cycle changes, histone H2AX phosphorylation (γ-H2AX) and DNA damage were evaluated in human alveolar epithelial cells at 24 h after exposure. Overall, HVOF particles were the most cytotoxic to human alveolar cells, with cell viability half-maximal inhibitory concentration (IC50) values of 20.18 µg/cm2 and 1.79 µg/cm2 for PGFP and PGNP, respectively. Only the highest tested concentration of APS-PGFP caused a slight decrease in cell viability. Particle uptake, cell cycle arrest at S + G2/M and γ-H2AX augmentation were observed after exposure to all tested particles. However, higher levels of γ-H2AX were found in cells exposed to APS-derived particles (~16%), while cells exposed to HVOF particles exhibited increased levels of oxidative damage (~17% tail intensity) and ROS (~184%). Accordingly, APS and HVOF particles seem to exert their genotoxic effects by different mechanisms, highlighting that the health risks of these process-generated particles at industrial settings should not be underestimated.pt_PT
dc.description.sponsorshipThis research was funded by the project CERASAFE with the support of ERA-NET SIINN (project id:16) and the Portuguese Foundation for Science and Technology (FCT; SIINN/0004/2014). This work was also supported by the project NanoBioBarriers (PTDC/MED-TOX/31162/2017), co financed by the Operational Program for Competitiveness and Internationalization (POCI) through European Regional Development Funds (FEDER/FNR) and FCT and the Spanish Ministry of Science and Innovation (projects PCIN-2015-173-C02-01 and CEX2018-000794-S-Severo Ochoa). M.J. Bessa (SFRH/BD/120646/2016) and F. Brandão (SFRH/BD/101060/2014) are recipients of FCT PhD scholarships under the framework of the Human Capital Operating Program (POCH) and European Union funding. The Doctoral Program in Biomedical Sciences of the ICBAS—University of Porto offered additional funds. S. Fraga thanks FCT for funding through program DL 57/2016—Norma transitória (Ref. DL-57/INSA-06/2018). Thanks are also due to FCT/MCTES for the financial support to EPIUnit and ITR (UIDB/04750/2020 and LA/P/0064/2020).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationInt J Mol Sci. 2022 Apr 13;23(8):4278. doi: 10.3390/ijms23084278pt_PT
dc.identifier.doi10.3390/ijms23084278pt_PT
dc.identifier.issn1661-6596
dc.identifier.urihttp://hdl.handle.net/10400.18/8451
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationSAFE PRODUCTION AND USE OF NANOMATERIALS IN THE CERAMIC INDUSTRY
dc.relationAssessing the toxicity of airborne nanoceramics in vitro and in vivo- contribution to the safe production and use of nanomaterials in the ceramic industry
dc.relationEstablishing grouping strategies according to toxicity and biological effects for silica based manufactured nanomaterials
dc.relationEpidemiology Research Unit - Institute of Public Health, University of Porto
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/23/8/4278pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectA549pt_PT
dc.subjectCell Cyclept_PT
dc.subjectCytotoxicitypt_PT
dc.subjectDNA Damagept_PT
dc.subjectIn vitro Toxicitypt_PT
dc.subjectIncidental Nanoparticlespt_PT
dc.subjectProcess-generated Nanoparticelspt_PT
dc.subjectOccupational Exposurept_PT
dc.subjectToxicologiapt_PT
dc.subjectAr e Saúde Ocupacionalpt_PT
dc.titleUnveiling the Toxicity of Fine and Nano-Sized Airborne Particles Generated from Industrial Thermal Spraying Processes in Human Alveolar Epithelial Cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleSAFE PRODUCTION AND USE OF NANOMATERIALS IN THE CERAMIC INDUSTRY
oaire.awardTitleAssessing the toxicity of airborne nanoceramics in vitro and in vivo- contribution to the safe production and use of nanomaterials in the ceramic industry
oaire.awardTitleEstablishing grouping strategies according to toxicity and biological effects for silica based manufactured nanomaterials
oaire.awardTitleEpidemiology Research Unit - Institute of Public Health, University of Porto
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FMED-TOX%2F31162%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/SIINN%2F0004%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F120646%2F2016/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F101060%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04750%2F2020/PT
oaire.citation.issue8pt_PT
oaire.citation.startPage4278pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume23pt_PT
oaire.fundingStream9471 - RIDTI
oaire.fundingStream3599-PPCDT
oaire.fundingStreamOE
oaire.fundingStream6817 - DCRRNI ID
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.nameFundação para a Ciência e a Tecnologia
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project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
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