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RND Efflux Systems Contribute to Resistance and Virulence of Aliarcobacter butzleri

2021-07-06Journal article Open access
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dc.contributor.authorMateus, Cristiana
dc.contributor.authorNunes, Ana Rita
dc.contributor.authorOleastro, Mónica
dc.contributor.authorDomingues, Fernanda
dc.contributor.authorFerreira, Susana
dc.date.accessioned2022-07-03T15:45:15Z
dc.date.available2022-07-03T15:45:15Z
dc.date.issued2021-07-06
dc.description(This article belongs to the Special Issue The Structural and Functional Study of Efflux Pumps Belonging to the RND Transporters Family from Gram-Negative Bacteria)pt_PT
dc.description.abstractAliarcobacter butzleri is an emergent enteropathogen that can be found in a range of environments. This bacterium presents a vast repertoire of efflux pumps, such as the ones belonging to the resistance nodulation cell division family, which may be associated with bacterial resistance, as well as virulence. Thus, this work aimed to evaluate the contribution of three RND efflux systems, AreABC, AreDEF and AreGHI, in the resistance and virulence of A. butzleri. Mutant strains were constructed by inactivation of the gene that encodes the inner membrane protein of these systems. The bacterial resistance profile of parental and mutant strains to several antimicrobials was assessed, as was the intracellular accumulation of the ethidium bromide dye. Regarding bacterial virulence, the role of these three efflux pumps on growth, strain fitness, motility, biofilm formation ability, survival in adverse conditions (oxidative stress and bile salts) and human serum and in vitro adhesion and invasion to Caco-2 cells was evaluated. We observed that the mutants from the three efflux pumps were more susceptible to several classes of antimicrobials than the parental strain and presented an increase in the accumulation of ethidium bromide, indicating a potential role of the efflux pumps in the extrusion of antimicrobials. The mutant strains had no bacterial growth defects; nonetheless, they presented a reduction in relative fitness. For the three mutants, an increase in the susceptibility to oxidative stress was observed, while only the mutant for AreGHI efflux pump showed a relevant role in bile stress survival. All the mutant strains showed an impairment in biofilm formation ability, were more susceptible to human serum and were less adherent to intestinal epithelial cells. Overall, the results support the contribution of the efflux pumps AreABC, AreDEF and AreGHI of A. butzleri to antimicrobial resistance, as well as to bacterial virulence.pt_PT
dc.description.sponsorshipThis work was financed by the Foundation for Science and Technology (FCT), through funds from the State Budget, and by the European Regional Development Fund (ERDF), under the Portugal 2020 Program, through the Regional Operational Program of the Center (Centro2020), through the Project with the reference UIDB/00709/2020. Cristiana Mateus is recipient of a doctoral fellowship (UI/BD/151023/2021) under the scope of the CICS-UBI Programmatic Funding (UIDP/00709/2020). Susana Ferreira acknowledges UBI and FCT by the contract of Scientific Employment according to DL57/2016.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAntibiotics (Basel). 2021 Jul 6;10(7):823. doi: 10.3390/antibiotics10070823pt_PT
dc.identifier.doi10.3390/antibiotics10070823pt_PT
dc.identifier.issn2079-6382
dc.identifier.urihttp://hdl.handle.net/10400.18/8048
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationHealth Sciences Research Centre
dc.relationUnravelling the virulence potential of Aliarcobacter butzleri
dc.relationHealth Sciences Research Centre
dc.relationNot Available
dc.relation.publisherversionhttps://www.mdpi.com/2079-6382/10/7/823pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAliarcobacter butzleript_PT
dc.subjectRND Efflux Pumpspt_PT
dc.subjectResistancept_PT
dc.subjectVirulencept_PT
dc.subjectInfecções Gastrointestinaispt_PT
dc.titleRND Efflux Systems Contribute to Resistance and Virulence of Aliarcobacter butzleript_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleUnravelling the virulence potential of Aliarcobacter butzleri
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//UI%2FBD%2F151023%2F2021/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/DL 57%2F2016/DL57%2F2016%2FCP1334%2FCP1644%2FCT0001/PT
oaire.citation.issue7pt_PT
oaire.citation.startPage823pt_PT
oaire.citation.titleAntibioticspt_PT
oaire.citation.volume10pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamDL 57/2016
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationced21566-cfde-4640-afff-0536a07c1f8f
relation.isProjectOfPublication60f6a70b-110d-4c15-a3fd-d43d078078bc
relation.isProjectOfPublicatione0fbaa7c-391a-479d-9bcf-efb7997d4004
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relation.isProjectOfPublication.latestForDiscoveryced21566-cfde-4640-afff-0536a07c1f8f

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