Repository logo
 
Publication

Genome-Scale Characterization of Mycobacterium abscessus Complex Isolates from Portugal

2023-10-20Journal article Open access
Simple item page
dc.contributor.authorCarneiro, Sofia
dc.contributor.authorPinto, Miguel
dc.contributor.authorSilva, Sónia
dc.contributor.authorSantos, Andrea
dc.contributor.authorRodrigues, Irene
dc.contributor.authorSantos, Daniela
dc.contributor.authorDuarte, Sílvia
dc.contributor.authorVieira, Luís
dc.contributor.authorGomes, João Paulo
dc.contributor.authorMacedo, Rita
dc.date.accessioned2024-02-12T15:24:23Z
dc.date.available2024-02-12T15:24:23Z
dc.date.issued2023-10-20
dc.description(This article belongs to the Special Issue Advances in Molecular Biology on Mycobacteria)pt_PT
dc.description.abstractThe Mycobacterium abscessus complex (MABC) is an emerging, difficult to treat, multidrug-resistant nontuberculous mycobacteria responsible for a wide spectrum of infections and associated with an increasing number of cases worldwide. Dominant circulating clones (DCCs) of MABC have been genetically identified as groups of strains associated with higher prevalence, higher levels of antimicrobial resistance, and worse clinical outcomes. To date, little is known about the genomic characteristics of MABC species circulating in Portugal. Here, we examined the genetic diversity and antimicrobial resistance profiles of 30 MABC strains isolated between 2014 and 2022 in Portugal. The genetic diversity of circulating MABC strains was assessed through a gene-by-gene approach (wgMLST), allowing their subspecies differentiation and the classification of isolates into DCCs. Antimicrobial resistance profiles were defined using phenotypic, molecular, and genomic approaches. The majority of isolates were resistant to at least two antimicrobials, although a poor correlation between phenotype and genotype data was observed. Portuguese genomes were highly diverse, and data suggest the existence of MABC lineages with potential international circulation or cross-border transmission. This study highlights the genetic diversity and antimicrobial resistance profile of circulating MABC isolates in Portugal while representing the first step towards the implementation of a genomic-based surveillance system for MABC at the Portuguese NIH.pt_PT
dc.description.sponsorshipFunding: The acquisition of equipment for whole-genome sequencing used in this study was funded by the HERA (Human and Environmental Risk Assessment) project (Grant/2021/PHF/23776) supported by the European Commission through the European Centre for Disease Prevention and Control (ECDC). This work was also partially funded by the GenomePT project (POCI-01-0145-FEDER-022184) supported by COMPETE 2020 (Operational Programme for Competitiveness and Internationalisation (POCI)), Lisboa Portugal Regional Operational Programme (Lisboa2020), Algarve Portugal Regional Operational Programme (CRESC Algarve2020) under the PORTUGAL 2020 Partnership Agreement through the European Regional Development Fund (ERDF), and the Portuguese Science and Technology Foundation (FCT). SC was supported by a PhD fellowship from the FCT (2020.08503.BD).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationInt J Mol Sci. 2023 Oct 20;24(20):15402. doi: 10.3390/ijms242015402pt_PT
dc.identifier.doi10.3390/ijms242015402pt_PT
dc.identifier.issn1661-6596
dc.identifier.urihttp://hdl.handle.net/10400.18/9087
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationNational Facility for genome sequencing and analysis
dc.relationGenomics of nontuberculous mycobacteria NTM: development of a laboratory tool for rapid and precise diagnosis and surveillance of NTM infections
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/24/20/15402pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMABCpt_PT
dc.subjectMycobacterium abscessuspt_PT
dc.subjectPortugalpt_PT
dc.subjectAntimicrobial Resistancept_PT
dc.subjectGenomic Diversitypt_PT
dc.subjectNontuberculous Mycobacteriapt_PT
dc.subjectwgMLSTpt_PT
dc.subjectwhole-genome Sequencingpt_PT
dc.subjectResistência aos Antimicrobianospt_PT
dc.titleGenome-Scale Characterization of Mycobacterium abscessus Complex Isolates from Portugalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNational Facility for genome sequencing and analysis
oaire.awardTitleGenomics of nontuberculous mycobacteria NTM: development of a laboratory tool for rapid and precise diagnosis and surveillance of NTM infections
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9444 - RNIIIE/PINFRA%2F22184%2F2016/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//2020.08503.BD/PT
oaire.citation.issue20pt_PT
oaire.citation.startPage15402pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume24pt_PT
oaire.fundingStream9444 - RNIIIE
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com a política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication3b8a803c-37e5-4354-93f5-6410d7fb2af7
relation.isProjectOfPublicationc849cd0a-1c17-4880-a44d-85567f9bce64
relation.isProjectOfPublication.latestForDiscoveryc849cd0a-1c17-4880-a44d-85567f9bce64

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
2023 - Carneiro et al (IJMS).pdf
Size:
3.33 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

DDI - Artigos em revistas internacionais
DGH - Artigos em revistas internacionais