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Prolonged exposure of Stenotrophomonas maltophilia biofilms to trace levels of clofibric acid alters antimicrobial tolerance and virulence

2019-11Journal article Restricted access
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dc.contributor.authorGomes, I.B.
dc.contributor.authorQuerido, M.M.
dc.contributor.authorTeixeira, J.P.
dc.contributor.authorPereira, C.C.
dc.contributor.authorSimões, L.C.
dc.contributor.authorSimões, M.
dc.date.accessioned2020-05-09T11:07:46Z
dc.date.available2020-05-09T11:07:46Z
dc.date.issued2019-11
dc.description.abstractThe presence of pharmaceuticals in water sources, including in drinking water (DW), is increasingly being recognized as an emerging and global concern for the environment and public health. Based on the principles of the "One Health" initiative, the present work aims to understand the effects of clofibric acid (CA), a lipid regulator, on the behavior of a selected bacterium isolated from drinking water (DW). Biofilms of the opportunistic pathogen Stenotrophomonas maltophilia were exposed to CA for 12 weeks at 170 and 17000 ng/L. The effects of CA were evaluated on planktonic S. maltophilia susceptibility to chlorine and antibiotics (amoxicillin, ciprofloxacin, erythromycin, kanamycin, levofloxacin, oxacillin, spectinomycin, tetracycline and trimethoprim-sulfamethoxazole), biofilm formation, motility, siderophores production and on the adhesion and internalization of the human colon adenocarcinoma cell line (HT-29). It was found that CA did not affect planktonic S. maltophilia tolerance to chlorine exposure. Additionally, no effects were observed on biofilm formation, motility and siderophores production. However, biofilms formed after CA exposure were more tolerant to chlorine disinfection and lower CFU reductions were obtained. Of additional concern was the effect of CA exposure on S. maltophilia increased tolerance to erythromycin. CA exposure also slightly reduced S. maltophilia ability to invade HT-29 cells. In conclusion, this work reinforces the importance of studying the effects of non-antibiotic contaminants on the behavior of environmental microorganisms, particularly their role as drivers affecting resistance evolution and selection.pt_PT
dc.description.abstractHighlights: Non-antibiotic drugs, including clofibric acid (CA) can affect bacterial behavior; The presence of CA in water increased S. maltophilia tolerance to erythromycin; CA exposure increased biofilm tolerance to chorine; CA exposure decreased S. maltophilia ability to adhere and internalize HT-29 cells.pt_PT
dc.description.sponsorshipThis work was financially supported by: project UID/EQU/ 00511/2019 - Laboratory for Process Engineering, Environment, Biotechnology and Energy e LEPABE funded by national funds through FCT/MCTES (PIDDAC); Projects POCI-01-0145-FEDER- 030219, POCI-01-0145-FEDER-028397 and POCI-01-0247-FEDER- 035234 funded by FEDER funds through COMPETE2020 e Programa Operacional Competitividade e Internacionalizaç~ao (POCI) and by national funds (PIDDAC) through FCT/MCTES; Project “LEPABE-2-ECO-INNOVATION” e NORTE-01-0145-FEDER-000005, funded by Norte Portugal Regional Operational Programme (NORTE, 2020), under PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). Grants attributed by Portuguese Foundation for Science and Technology e FCT e to In^es Gomes (SFRH/BD/103810/2014) and to Micaela Machado Querido (SFRH/BD/130203/2017).
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationChemosphere. 2019 Nov;235:327-335. doi: 10.1016/j.chemosphere.2019.06.184. Epub 2019 Jun 25pt_PT
dc.identifier.doi10.1016/j.chemosphere.2019.06.184pt_PT
dc.identifier.issn0045-6535
dc.identifier.urihttp://hdl.handle.net/10400.18/6626
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier/ Pergamonpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0045653519314250?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectAnti-Bacterial Agentspt_PT
dc.subjectAnti-Infective Agentspt_PT
dc.subjectBiofilmspt_PT
dc.subjectCiprofloxacinpt_PT
dc.subjectClofibric Acidpt_PT
dc.subjectDrinking Waterpt_PT
dc.subjectDrug Tolerancept_PT
dc.subjectHT29 Cellspt_PT
dc.subjectHumanspt_PT
dc.subjectStenotrophomonas maltophiliapt_PT
dc.subjectVirulencept_PT
dc.subjectÁgua e Solopt_PT
dc.titleProlonged exposure of Stenotrophomonas maltophilia biofilms to trace levels of clofibric acid alters antimicrobial tolerance and virulencept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage335pt_PT
oaire.citation.startPage327pt_PT
oaire.citation.titleChemospherept_PT
oaire.citation.volume235pt_PT
rcaap.embargofctDe acordo com política editorial da revista.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

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