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Maternal dietary exposure to mycotoxin aflatoxin B1 promotes intestinal immune alterations and microbiota modifications increasing infection susceptibility in mouse offspring

2023-01-02Journal article Restricted access
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dc.contributor.authorBastos-Amador, Patricia
dc.contributor.authorDuarte, Elsa Leclerc
dc.contributor.authorTorres, Júlio
dc.contributor.authorCaldeira, Ana Teresa
dc.contributor.authorSilva, Inês
dc.contributor.authorSalvador, Cátia
dc.contributor.authorAssunção, Ricardo
dc.contributor.authorAlvito, Paula
dc.contributor.authorFerreira, Manuela
dc.date.accessioned2023-10-06T10:50:12Z
dc.date.available2023-10-06T10:50:12Z
dc.date.issued2023-01-02
dc.description.abstractAbstract: Mycotoxins are secondary metabolites produced by fungi occurring in food that are toxic to animals and humans. Early-life mycotoxins exposure has been linked to diverse pathologies. However, how maternal exposure to mycotoxins impacts on the intestinal barrier function of progeny has not been explored. Here, exposure of pregnant and lactating C57Bl/6J female mice to aflatoxin B1 (AFB1; 400 μg/kg body weight/day; 3 times a week) in gelatine pellets, from embryonic day (E)11.5 until weaning (postnatal day 21), led to gut immunological changes in progeny. The results showed an overall increase of lymphocyte number in intestine, a reduction of expression of epithelial genes related to microbial defence, as well as a decrease in cytokine production by intestinal type 2 innate lymphoid cells (ILC2). While susceptibility to chemically induced colitis was not worsened, immune alterations were associated with changes in gut microbiota and with a higher vulnerability to infection by the protozoan Eimeria vermiformis at early-life. Together these results show that maternal dietary exposure to AFB1 can dampen intestinal barrier homeostasis in offspring decreasing their capability to tackle intestinal pathogens. These data provide insights to understand AFB1 potential harmfulness in early-life health in the context of intestinal infections.pt_PT
dc.description.abstractHighlights: Effect of maternal AFB1 dietary exposure was studied in intestinal mucosa of offspring mice; Maternal AFB1 exposure leads to lymphocyte expansion and alters cytokine expression; Maternal AFB1 exposure reduces microbial-related epithelial gene expression in offspring; Maternal AFB1 exposure modifies offspring gut microbiota.; Maternal AFB1-exposed offspring are more vulnerable to intestinal parasite infection.pt_PT
dc.description.sponsorshipEarlyMyco (PTDC/MED-TOX/28762/ 2017), investigator FCT Exploratory (IF/00425/2015/CP1324/CT0001) and CESAM (UIDP/50017/2020+UIDB/50017/2020+LA/P/0094/ 2020)pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFood Chem Toxicol. 2023 Mar:173:113596. doi: 10.1016/j.fct.2022.113596. Epub 2023 Jan 2.pt_PT
dc.identifier.doi10.1016/j.fct.2022.113596pt_PT
dc.identifier.issn0278-6915
dc.identifier.urihttp://hdl.handle.net/10400.18/8663
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationRole of diet-derived retinoids in natural intraepithelial lymphocytes and intestinal defence
dc.relationCentre for Environmental and Marine Studies
dc.relationCentre for Environmental and Marine Studies
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0278691522007943?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectMycotoxinspt_PT
dc.subjectEarly-life Exposurept_PT
dc.subjectMaternal Dietary Exposurept_PT
dc.subjectIntestinal Immune Systempt_PT
dc.subjectMicotoxinaspt_PT
dc.subjectMicrobiotapt_PT
dc.subjectExposição Precocept_PT
dc.subjectSegurança Alimentarpt_PT
dc.subjectToxicologiapt_PT
dc.titleMaternal dietary exposure to mycotoxin aflatoxin B1 promotes intestinal immune alterations and microbiota modifications increasing infection susceptibility in mouse offspringpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleRole of diet-derived retinoids in natural intraepithelial lymphocytes and intestinal defence
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMED-TOX%2F28762%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00425%2F2015%2FCP1324%2FCT0001/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50017%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50017%2F2020/PT
oaire.citation.startPage113596pt_PT
oaire.citation.titleFood and Chemical Toxicologypt_PT
oaire.citation.volume173pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamInvestigador FCT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com a política editorial da revistapt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication2635814e-db0a-43c5-b745-a2a237efb72e
relation.isProjectOfPublication117df56d-0cdc-4967-a2e3-f4ee81b75d5c
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relation.isProjectOfPublication0e72e6c8-b610-43e0-931c-8ad275ad6093
relation.isProjectOfPublication.latestForDiscovery2635814e-db0a-43c5-b745-a2a237efb72e

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