Publicação
Bioremediation of Bacteria, Histamine and Animal DNA by Black Soldier Fly Larvae for Safe Food Waste Valorisation
| datacite.subject.sdg | 02:Erradicar a Fome | |
| datacite.subject.sdg | 12:Produção e Consumo Sustentáveis | |
| datacite.subject.sdg | 03:Saúde de Qualidade | |
| dc.contributor.author | Oliveira, Joana | |
| dc.contributor.author | Ligeiro, Carolina | |
| dc.contributor.author | Carvalho, Carina | |
| dc.contributor.author | Souza, Clarice | |
| dc.contributor.author | Grilo, Miguel L. | |
| dc.contributor.author | Fantatto, Rafaela | |
| dc.contributor.author | Trindade, Alexandre | |
| dc.contributor.author | Murta, Daniel | |
| dc.contributor.author | Assunção, Ricardo | |
| dc.date.accessioned | 2026-02-10T10:12:18Z | |
| dc.date.available | 2026-02-10T10:12:18Z | |
| dc.date.issued | 2025-10-28 | |
| dc.description | Comunicação realizada na forma de "pitch". | |
| dc.description.abstract | By 2050, the global population is expected to reach 9.8 billion, increasing pressure on agri-food systems and worsening food waste. In the European Union (EU), around 59 million tonnes of food are wasted annually, enough to feed 1.26 hungry billion people per year. This waste has major public health impacts. Tackling it through safe, sustainable strategies is, therefore, essential. Black soldier fly larvae (BSFL; Hermetia illucens) offer a promising solution by converting food waste into high-value products like protein, fat, and organic fertiliser. However, EU legislation currently prohibits using food waste as insect feed due to possible contamination with prions, and other foodborne hazards such as bacteria and histamine. This study evaluates the food safety potential of BSFL by assessing their ability to reduce pathogenic bacteria and histamine, and to determine whether they bioaccumulate animal DNA (pork, beef, chicken). To test this, 2 tonnes of heterogeneous food waste were transformed into BSFL substrate and used in a bioconversion assay. Larvae in the test group showed improved performance, with higher bioconversion and growth rates and a lower feed conversion ratio compared to the control group. Analyses were conducted on food waste substrate, larvae, BSFL meal, and frass, including controls. Bacterial enumeration followed ISO protocols; histamine was quantified using an ELISA kit; DNA was extracted and then analysed via PCR and agarose gel electrophoresis.BSFL significantly reduced Salmonella spp. and Vibrio spp. in the test group (p = 0.002), and Bacillus cereus and Vibrio spp. in the control (p = 0.015). Histamine levels decreased significantly (p = 0.029). No animal DNA was detected in larvae (p = 0.029), though traces persisted in frass, indicating excretion rather than bioaccumulation. These findings support the safe use of BSFL in food waste valorisation and their integration into circular, One Health food systems focused on safety and sustainability. | eng |
| dc.description.sponsorship | “InsectERA” (Project No. C644917393-00000032) within the WPs InBioremediation and One Health, funded by Next Generation EU European Fund and the Portuguese Recovery and Resilience Plan (PRR), under the scope of the incentive line “Agendas for Business Innovation” through the funding scheme C5—Capitalization and Business Innovation | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/10876 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.relation | A Mosca Soldado Negro como ferramenta de Biorremediação: uma perspetiva One Health./ The Black Soldier Fly as a bioremediation instrument: a One Heath perspective. | |
| dc.relation.hasversion | https://sciforum.net/paper/view/25135 | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Insects | |
| dc.subject | Food Safety | |
| dc.subject | Sustainable Agri-Food Systems | |
| dc.subject | One Health | |
| dc.subject | Segurança Alimentar | |
| dc.subject | Avaliação do Risco | |
| dc.subject | Nutrição Aplicada | |
| dc.subject | Toxicologia | |
| dc.title | Bioremediation of Bacteria, Histamine and Animal DNA by Black Soldier Fly Larvae for Safe Food Waste Valorisation | eng |
| dc.type | conference object | |
| dspace.entity.type | Publication | |
| oaire.awardNumber | 2022.13540.BDANA | |
| oaire.awardTitle | A Mosca Soldado Negro como ferramenta de Biorremediação: uma perspetiva One Health./ The Black Soldier Fly as a bioremediation instrument: a One Heath perspective. | |
| oaire.awardURI | http://hdl.handle.net/10400.18/10652 | |
| oaire.citation.conferenceDate | 2025-10-28 | |
| oaire.citation.conferencePlace | Online | |
| oaire.citation.title | 6th International Electronic Conference on Foods, 28-30 October 2025 | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |
| relation.isProjectOfPublication | 52a0447b-726d-4d7f-9283-031c7475df19 | |
| relation.isProjectOfPublication.latestForDiscovery | 52a0447b-726d-4d7f-9283-031c7475df19 |