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Investigating ingested nanomaterials’ safety – the case of TiO2 and innovative nanocelluloses.

2023-09-04Conference object Restricted access
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dc.contributor.authorVital, Nádia
dc.contributor.authorSilva, Maria João
dc.contributor.authorLouro, Henriqueta
dc.date.accessioned2024-02-15T15:13:33Z
dc.date.available2024-02-15T15:13:33Z
dc.date.issued2023-09-04
dc.descriptionThanks to P. Ferreira, J.Pedrosa (University of Coimbra) and A.F.Lourenço (RAIZ) for the CMNs; to P.Alvito, R.Assunção, M.Silva (Food Safety Department, INSA) for in vitro digestion.pt_PT
dc.description.abstractIntroduction: The development of nanomaterials(NMs)-based technologies led to their increased use in key sectors and products related to food, food contact materials and feed. Many available products have NMs, as intentional constituents or contaminants from process ou food packaging release, such as silicon or titanium dioxide(TiO2) NMs. Others are being developed, like nanocelluloses(CNMs; doi:10.3390/nano12193375). However, it is recognised that the NMs’ specific physicochemical properties, conferring them unique benefi cial characteristics, can also elicit nano-bio interactions leading to toxic potential. Also, their dynamic behaviour in the surrounding matrix, may lead to secondary features determining the toxicological outcomes. Recognizing that processes like intake or digestion may modify the NMs’ characteristics leading to unexpected toxicity in human cells, EFSA included the use of in vitro digestion models in their specific guidelines concerning risk assessment of nanomaterials for food and feed(DOI:10.2903/j.efsa.2021.6768). Methodology: With the aim to contribute to the safety assessment of NMs, intestinal cell models (Caco-2 and HT29-MTX-E12 cells) were exposed to TiO2 NMs or innovative CNMs. Additionally, samples submitted previously to in vitro simulation of human digestion were used, and the genotoxicity(comet and micronucleus assays) was investigated with and without the digestion process. Results: After TiO2 NMs’ exposure, the micronucleus assay, an indicator of cancer risk, suggested eff ects on the chromosomal integrity in the HT29-MTX-E12 cells, for all the tested TiO2 NMs, especially after the in vitro digestion. Upon exposure to the two CNMs, no chromosomal damage was observed in the micronucleus assay, but the comet assay revealed DNA damage in the same cells, after 3h and 24h exposure, an effect slightly more relevant after the digestion of the cellulose nanofibril. Conclusion: Overall, the results show diff erent outcomes when using different NMs, and with/without digestion. Thus, it is important to consider the primary and secondary NMs’ characteristics determining the adverse eff ects, taking into account the human digestion for nanosafety assessment.pt_PT
dc.description.sponsorshipFunded by FCT/MCTES through 2020.07168.BD (NV), PTDC/SAUPUB/29481/2017, and ToxOmics (UIDB/00009/2020;UIDP/00009/2020).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/9116
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationToxicological profile of cellulose nanomaterials in human gastrointestinal cells
dc.relationCellular and Molecular Mechanisms of Toxicity of Ingested Nanomaterials
dc.relationCentre for Toxicogenomics and Human Health
dc.relationCentre for Toxicogenomics and Human Health
dc.subjectEnvironmental Genotoxicitypt_PT
dc.subjectIngested Nanomaterialspt_PT
dc.subjectNanocellulosept_PT
dc.subjectTitanium Dioxidept_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleInvestigating ingested nanomaterials’ safety – the case of TiO2 and innovative nanocelluloses.pt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleToxicological profile of cellulose nanomaterials in human gastrointestinal cells
oaire.awardTitleCellular and Molecular Mechanisms of Toxicity of Ingested Nanomaterials
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//2020.07168.BD/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso para Financiamento de Projetos de Investigação Científica e Desenvolvimento Tecnológico em Todos os Domínios Científicos - 2017/PTDC%2FSAU-PUB%2F29481%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00009%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00009%2F2020/PT
oaire.citation.conferencePlaceCampinas, Brasil/Onlinept_PT
oaire.citation.title5th edition of the International Conference on Food Contaminants (ICFC 2023) 'Challenges on exposure assessment, health impact and sustainability of food systems', 4-6 julho 2023pt_PT
oaire.fundingStreamConcurso para Financiamento de Projetos de Investigação Científica e Desenvolvimento Tecnológico em Todos os Domínios Científicos - 2017
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameVital
person.familyNameSilva
person.familyNameLouro
person.givenNameNádia
person.givenNameMaria Joao
person.givenNameHenriqueta
person.identifier157627
person.identifier.ciencia-id7710-643D-97A3
person.identifier.ciencia-id721D-2BB1-7DB1
person.identifier.orcid0000-0002-3391-0268
person.identifier.orcid0000-0002-6060-0716
person.identifier.orcid0000-0001-9744-7332
person.identifier.scopus-author-id55944437600
person.identifier.scopus-author-id6507971479
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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