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Phylogenetic Evidence for the Existence of Multiple Strains of Rickettsia parkeri in the New World

2018-04-02Journal article Open access
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dc.contributor.authorNieri-Bastos, F.A.
dc.contributor.authorMarcili, A.
dc.contributor.authorde Sousa, R.
dc.contributor.authorPaddock, C.D.
dc.contributor.authorLabruna, M.B.
dc.date.accessioned2019-03-04T15:38:40Z
dc.date.available2019-03-04T15:38:40Z
dc.date.issued2018-04-02
dc.descriptionFree PMC Article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5881050/pt_PT
dc.description.abstractThe bacterium Rickettsia parkeri has been reported to infect ticks of the "Amblyomma maculatum species complex" in the New World, where it causes spotted fever illness in humans. In South America, three additional rickettsial strains, namely, Atlantic rainforest, NOD, and Parvitarsum, have been isolated from the ticks Amblyomma ovale, Amblyomma nodosum, and Amblyomma parvitarsum, respectively. These three strains are phylogenetically closely related to R. parkeri, Rickettsia africae, and Rickettsia sibirica Herein, we performed a robust phylogenetic analysis encompassing 5 genes (gltA, ompA, virB4, dnaA, and dnaK) and 3 intergenic spacers (mppE-pur, rrl-rrf-ITS, and rpmE-tRNAfMet) from 41 rickettsial isolates, including different isolates of R. parkeri, R. africae, R. sibirica, Rickettsia conorii, and strains Atlantic rainforest, NOD, and Parvitarsum. In our phylogenetic analyses, all New World isolates grouped in a major clade distinct from the Old World Rickettsia species (R. conorii, R. sibirica, and R. africae). This New World clade was subdivided into the following 4 clades: the R. parkerisensu stricto clade, comprising the type strain Maculatum 20 and all other isolates of R. parkeri from North and South America, associated with ticks of the A. maculatum species complex; the strain NOD clade, comprising two South American isolates from A. nodosum ticks; the Parvitarsum clade, comprising two South American isolates from A. parvitarsum ticks; and the strain Atlantic rainforest clade, comprising six South American isolates from the A. ovale species complex (A. ovale or Amblyomma aureolatum). Under such evidences, we propose that strains Atlantic rainforest, NOD, and Parvitarsum are South American strains of R. parkeriIMPORTANCE Since the description of Rickettsia parkeri infecting ticks of the "Amblyomma maculatum species complex" and humans in the New World, three novel phylogenetic close-related rickettsial isolates were reported in South America. Herein, we provide genetic evidence that these novel isolates, namely, strains Atlantic rainforest, NOD, and Parvitarsum, are South American strains of R. parkeri. Interestingly, each of these R. parkeri strains seems to be primarily associated with a tick species group, namely, R. parkerisensu stricto with the "Amblyomma maculatum species group," R. parkeri strain NOD with Amblyomma nodosum, R. parkeri strain Parvitarsum with Amblyomma parvitarsum, and R. parkeri strain Atlantic rainforest with the "Amblyomma ovale species group." Such rickettsial strain-tick species specificity suggests a coevolution of each tick-strain association. Finally, because R. parkerisensu stricto and R. parkeri strain Atlantic rainforest are human pathogens, the potential of R. parkeri strains NOD and Parvitarsum to be human pathogens cannot be discarded.pt_PT
dc.description.sponsorshipThis work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (grant 2011/51979-1 to F.A.N.-B.) and by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior CAPES/PROEX 1841/2016.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAppl Environ Microbiol. 2018 Apr 2;84(8). pii: e02872-17. doi: 10.1128/AEM.02872-17. Print 2018 Apr 15pt_PT
dc.identifier.doi10.1128/AEM.02872-17pt_PT
dc.identifier.issn0099-2240
dc.identifier.urihttp://hdl.handle.net/10400.18/6053
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Society for Microbiologypt_PT
dc.relation.publisherversionhttps://aem.asm.org/content/84/8/e02872-17.longpt_PT
dc.subjectRickettsiapt_PT
dc.subjectAmblyommapt_PT
dc.subjectNew Worldpt_PT
dc.subjectRickettsia Parkeript_PT
dc.subjectSpotted Fever Grouppt_PT
dc.subjectTick-borne Diseasespt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.titlePhylogenetic Evidence for the Existence of Multiple Strains of Rickettsia parkeri in the New Worldpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue8pt_PT
oaire.citation.startPagee02872-17pt_PT
oaire.citation.titleApplied and Environmental Microbiologypt_PT
oaire.citation.volume84pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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