Campylobacter spp. in Portuguese fresh meat: use of  filter membranes for the detection in food and resistance profile of the isolate

Furtado, Rosália; Belo Correia, Cristina; Varela Flores, Cristina; Saraiva, Margarida; Santos, Andrea; Rodrigues, João Carlos; Oleastro, Mónica; Calhau, Maria Antónia

http://hdl.handle.net/10400.18/2514

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Authors

Furtado, Rosália

Belo Correia, Cristina

Varela Flores, Cristina

Saraiva, Margarida

Santos, Andrea

Rodrigues, João Carlos

Oleastro, Mónica

Calhau, Maria Antónia

Abstract(s)

Campylobacteriosis is considered the main cause of acute gastroenteritis in the European Union with a continuous increase in reported cases over the last five years. Due to the global increase of antibiotics use in food animals production, antibiotic resistance has became a significant threat and a public health problem. As a consequence the number of humans cases infected by multiresistant strains of Campylobacter is growing. The aim of this study was to evaluate an alternative plating media for isolation of Campylobacter spp and determine the antibiotic resistance of the isolates. According to ISO 10272-1:2006 protocol plating, two different agars are specified, being the use of a mCCDA-based medium mandatory. Therefore, two plating techniques were used in parallel: plating in mCCDA medium and the filtration technique on the surface of Columbia Blood agar. In addition, a Karmali agar or a chromogenic agar was also used. During 2012/2013, a total of 184 samples of fresh meat were analyzed and a total of 37 isolates were recovered: 30 C. coli, 7 C. jejuni. The filtration technique provided the highest number of positive results for Campylobacter spp and reduced the interference caused by the background flora, preventing the occurrence of false negative results. It showed to be a simple technique, with an improved rate of isolation and recover of this pathogen, and reduced labor and costs. All isolates were tested for ciprofloxacin, tetracycline and erythromycin resistance, using the disc diffusion method. A high rate of resistance to these antibiotics was found. We also highlight the presence of multiresistant Campylobacter in food of animal origin to antibiotics used in human therapeutic. These results increase the importance of strengthening an integrated epidemiological surveillance of campylobacteriosis, involving the areas of animal health, food production and human health.