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Guidelines for the Direct Detection of Anaplasma spp. in Diagnosis and Epidemiological Studies

2017-01Journal article Restricted access
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dc.contributor.authorSilaghi, C.
dc.contributor.authorSantos, A.S.
dc.contributor.authorGomes, J.
dc.contributor.authorChristova, I.
dc.contributor.authorMatei, I.A.
dc.contributor.authorWalder, G.
dc.contributor.authorDomingos, A.
dc.contributor.authorBell-Sakyi, L.
dc.contributor.authorSprong, H.
dc.contributor.authorvon Loewenich, F.D.
dc.contributor.authorOteo, J.A.
dc.contributor.authorde la Fuente, J.
dc.contributor.authorDumler, J.S.
dc.date.accessioned2018-02-01T15:42:11Z
dc.date.available2018-02-01T15:42:11Z
dc.date.issued2017-01
dc.description.abstractThe genus Anaplasma (Rickettsiales: Anaplasmataceae) comprises obligate intracellular Gram-negative bacteria that are mainly transmitted by ticks, and currently includes six species: Anaplasma bovis, Anaplasma centrale, Anaplasma marginale, Anaplasma phagocytophilum, Anaplasma platys, and Anaplasma ovis. These have long been known as etiological agents of veterinary diseases that affect domestic and wild animals worldwide. A zoonotic role has been recognized for A. phagocytophilum, but other species can also be pathogenic for humans. Anaplasma infections are usually challenging to diagnose, clinically presenting with nonspecific symptoms that vary greatly depending on the agent involved, the affected host, and other factors such as immune status and coinfections. The substantial economic impact associated with livestock infection and the growing number of human cases along with the risk of transfusion-transmitted infections, determines the need for accurate laboratory tests. Because hosts are usually seronegative in the initial phase of infection and serological cross-reactions with several Anaplasma species are observed after seroconversion, direct tests are the best approach for both case definition and epidemiological studies. Blood samples are routinely used for Anaplasma spp. screening, but in persistently infected animals with intermittent or low-level bacteremia, other tissues might be useful. These guidelines have been developed as a direct outcome of the COST action TD1303 EURNEGVEC ("European Network of Neglected Vectors and Vector-Borne Diseases"). They review the direct laboratory tests (microscopy, nucleic acid-based detection and in vitro isolation) currently used for Anaplasma detection in ticks and vertebrates and their application.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationVector Borne Zoonotic Dis. 2017 Jan;17(1):12-22.pt_PT
dc.identifier.doi10.1089/vbz.2016.1960pt_PT
dc.identifier.issn1530-3667
dc.identifier.urihttp://hdl.handle.net/10400.18/4902
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMary Ann Liebert/Society for Zoonotic Ecology and Epidemiology (SocZEE)pt_PT
dc.relation.publisherversionhttps://doi.org/10.1089/vbz.2016.1960pt_PT
dc.subjectAnaplasma spp.pt_PT
dc.subjectPCRpt_PT
dc.subjectDirect Diagnosispt_PT
dc.subjectIn vitro Isolationpt_PT
dc.subjectMicroscopypt_PT
dc.subjectTickspt_PT
dc.subjectVertebrate Hostspt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.titleGuidelines for the Direct Detection of Anaplasma spp. in Diagnosis and Epidemiological Studiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage22pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage12pt_PT
oaire.citation.titleVector-Borne and Zoonotic Diseasespt_PT
oaire.citation.volume17pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

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