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Publicação
Translational regulation by upstream open reading frames and human diseases
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Orientador(es)
Resumo(s)
Short upstream open reading frames (uORFs) are cis-acting elements located within the 5'-leader sequence of transcripts and are defined by an initiation codon in-frame with a termination codon located upstream or downstream of its main ORF (mORF) initiation codon. Recent genome-wide ribosome profiling studies have confirmed the widespread presence of uORFs and have shown that many uORFs can initiate with non-AUG codons. uORFs can impact gene expression of the downstream mORF by triggering mRNA decay or by regulating translation. Thus, disruption or creation of uORFs can elicit the development of several genetic diseases. Here, we review the mechanisms by which AUG- and non-AUG uORFs regulate translation. We also show some examples of uORF deregulation in human genetic diseases, focusing mainly on cancer. The knowledge of how uORF deregulation drives the onset of a disease, points out the need to screen the 5'-leader sequences of the transcripts in search for potential disease-related variants. This information will be relevant for the implementation of new diagnostic and/or therapeutic tools.
Descrição
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1157)
L. Romão (ed.), The mRNA Metabolism in Human Disease, Advances in Experimental Medicine and Biology 1157, https://doi.org/10.1007/978-3-030-19966-1_5
L. Romão (ed.), The mRNA Metabolism in Human Disease, Advances in Experimental Medicine and Biology 1157, https://doi.org/10.1007/978-3-030-19966-1_5
Palavras-chave
Genetic Disease Non-AUG Upstream Open Reading Frame (uORF) Stress Translational Regulation Translatome uORF uORF-encoded Peptide Doenças Genéticas Genómica Funcional e Estrutural
Contexto Educativo
Citação
Adv Exp Med Biol. 2019;1157:99-116. doi: 10.1007/978-3-030-19966-1_5
Editora
Springer Verlag