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Performance of Human Fecal Anaerobe-Associated PCR-Based Assays in a Multi-Laboratory Method Evaluation Study

2013-07-05Journal article Restricted access
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dc.contributor.authorLayton, Blythe
dc.contributor.authorYiping, Cao
dc.contributor.authorEbentier, Darcy
dc.contributor.authorKaitlyn, Hanley
dc.contributor.authorBallesté, Elisenda
dc.contributor.authorBrandão, João
dc.contributor.authorByappanahalli, Muruleedhara
dc.contributor.authorConverse, Reagan
dc.contributor.authorFarnleitner, Andreas
dc.contributor.authorGentry-Shields, Jennifer
dc.contributor.authorGidley, Maribeth
dc.contributor.authorGourmelon, Michèle
dc.contributor.authorSoo Lee, Chang
dc.contributor.authorLee, Jiyoung
dc.contributor.authorLozach, Solen
dc.contributor.authorMadi, Tania
dc.contributor.authorMeijer, Wim
dc.contributor.authorNoble, Rachel
dc.contributor.authorPeed, Lindsay
dc.contributor.authorReischer, Georg
dc.contributor.authorRodrigues, Raquel
dc.contributor.authorRose, Joan
dc.contributor.authorSchriewer, Alexander
dc.contributor.authorSinigalliano, Chris
dc.contributor.authorSrinivasan, Sangeetha
dc.contributor.authorStewart, Jill
dc.contributor.authorVan De Werfhorst, Laurie
dc.contributor.authorWang, Dan
dc.contributor.authorWhitman, Richard
dc.contributor.authorWuertz, Stefan
dc.contributor.authorJay, Jenny
dc.contributor.authorHolden, Patricia
dc.contributor.authorBoehm, Alexandria
dc.contributor.authorShanks, Orin
dc.contributor.authorGriffith, John
dc.date.accessioned2013-09-17T15:30:39Z
dc.date.available2013-09-17T15:30:39Z
dc.date.issued2013-07-05
dc.description.abstractA number of PCR-based methods for detecting human fecal material in environmental waters have been developed over the past decade, but these methods have rarely received independent comparative testing in large multi-laboratory studies. Here, we evaluated ten of these methods (BacH, BacHum-UCD, B. thetaiotaomicron (BtH), BsteriF1, gyrB, HF183 endpoint, HF183 SYBR, HF183 Taqman, HumM2, and M. smithii nifH (Mnif)) using 64 blind samples prepared in one laboratory. The blind samples contained either one or two fecal sources from human, wastewater or non-human sources. The assay results were assessed for presence/absence of the human markers and also quantitatively while varying the following: 1) classification of samples that were detected but not quantifiable (DNQ) as positive or negative; 2) reference fecal sample concentration unit of measure (such as culturable indicator bacteria, wet mass, total DNA, etc); and 3) human fecal source type (stool, sewage or septage). Assay performance using presence/absence metrics was found to depend on the classification of DNQ samples. The assays that performed best quantitatively varied based on the fecal concentration unit of measure and laboratory protocol. All methods were consistently more sensitive to human stools compared to sewage or septage in both the presence/absence and quantitative analysis. Overall, HF183 Taqman was found to be the most effective marker of human fecal contamination in this California-based study.por
dc.identifier.citationWater Res. 2013 Jul 5. pii: S0043-1354(13)00550-2. doi: 10.1016/j.watres.2013.05.060.por
dc.identifier.issn0043-1354
dc.identifier.otherdoi: 10.1016/j.watres.2013.05.060
dc.identifier.otherESSN: 1879-2448
dc.identifier.urihttp://hdl.handle.net/10400.18/1721
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0043135413005502por
dc.subjectMicrobial Source Trackingpor
dc.subjectBacteroidalespor
dc.subjectqPCRpor
dc.subjectWater Qualitypor
dc.subjectBacteroidespor
dc.titlePerformance of Human Fecal Anaerobe-Associated PCR-Based Assays in a Multi-Laboratory Method Evaluation Studypor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleWater Researchpor
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor

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