Synergy Between Pseudomonas aeruginosa Filtrates And Voriconazole Against Aspergillus fumigatus Biofilm Is Less for Mucoid Isolates From Persons With Cystic Fibrosis

Sass, Gabriele; Marsh, Julianne J.; Shrestha, Pallabi; Sabino, Raquel; Stevens, David A.

Publication

Synergy Between Pseudomonas aeruginosa Filtrates And Voriconazole Against Aspergillus fumigatus Biofilm Is Less for Mucoid Isolates From Persons With Cystic Fibrosis

2022-04-14Journal article

dc.contributor.author	Sass, Gabriele
dc.contributor.author	Marsh, Julianne J.
dc.contributor.author	Shrestha, Pallabi
dc.contributor.author	Sabino, Raquel
dc.contributor.author	Stevens, David A.
dc.date.accessioned	2023-02-08T11:07:27Z
dc.date.available	2023-02-08T11:07:27Z
dc.date.issued	2022-04-14
dc.description	This article is part of the Research Topic: Control of Human and Animal Aspergillosis – a Health Approach	pt_PT
dc.description.abstract	Persons with cystic fibrosis (CF) frequently suffer from Pseudomonas aeruginosa and Aspergillus fumigatus co-infections. There is evidence that co-infections with these interacting pathogens cause airway inflammation and aggravate deterioration of lung function. We recently showed that P. aeruginosa laboratory isolates synergistically interact with the anti-fungal azole voriconazole (VCZ), inhibiting biofilm metabolism of several A. fumigatus laboratory strains. Interaction was usually mediated via pyoverdine, but also via pyocyanin or pyochelin. Here we used planktonic filtrates of 7 mucoid and 9 non-mucoid P. aeruginosa isolates from CF patients, as well as 8 isolates without CF origin, and found that all of these isolates interacted with VCZ synergistically at their IC50 as well as higher dilutions. CF mucoid isolates showed the weakest interactive effects. Four non-mucoid P. aeruginosa CF isolates produced no or very low levels of pyoverdine and did not reach an IC50 against forming A. fumigatus biofilm; interaction with VCZ still was synergistic. A VCZ-resistant A. fumigatus strain showed the same level of susceptibility for P. aeruginosa anti-fungal activity as a VCZ-susceptible reference strain. Filtrates of most Pseudomonas isolates were able to increase anti-fungal activity of VCZ on a susceptible A. fumigatus strain. This was also possible for the VCZ-resistant strain. In summary these data show that clinical P. aeruginosa isolates, at varying degrees, synergistically interact with VCZ, and that pyoverdine is not the only molecule responsible. These data also strengthen the idea that during co-infections of A. fumigatus and P. aeruginosa lower concentrations of VCZ might be sufficient to control fungal growth.	pt_PT
dc.description.sponsorship	These studies were funded by ongoing support by the Foundation for Research in Infectious Diseases (FRID, grant 8201).	pt_PT
dc.description.version	info:eu-repo/semantics/publishedVersion	pt_PT
dc.identifier.citation	Front Cell Infect Microbiol. 2022 Apr 14;12:817315. doi: 10.3389/fcimb.2022.817315. eCollection 2022.	pt_PT
dc.identifier.doi	10.3389/fcimb.2022.817315	pt_PT
dc.identifier.issn	2235-2988
dc.identifier.uri	http://hdl.handle.net/10400.18/8509
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.publisher	Frontiers Media	pt_PT
dc.relation.publisherversion	https://www.frontiersin.org/articles/10.3389/fcimb.2022.817315/full	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	pt_PT
dc.subject	Pseudomonas Aeruginosa	pt_PT
dc.subject	Aspergillus Fumigatus	pt_PT
dc.subject	Voriconazole	pt_PT
dc.subject	Therapy	pt_PT
dc.subject	Microbial Interaction	pt_PT
dc.subject	Drug Interaction	pt_PT
dc.subject	Cystic Fibrosis	pt_PT
dc.subject	Infecções Sistémicas e Zoonoses	pt_PT
dc.title	Synergy Between Pseudomonas aeruginosa Filtrates And Voriconazole Against Aspergillus fumigatus Biofilm Is Less for Mucoid Isolates From Persons With Cystic Fibrosis	pt_PT
dc.type	journal article
dspace.entity.type	Publication
oaire.citation.startPage	817315	pt_PT
oaire.citation.title	Frontiers in Cellular and Infection Microbiology	pt_PT
oaire.citation.volume	12	pt_PT
rcaap.embargofct	Acesso de acordo com política editorial da revista.	pt_PT
rcaap.rights	openAccess	pt_PT
rcaap.type	article	pt_PT