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A retrospective cross-sectional quantitative molecular approach in biological samples from patients with syphilis

2017-03Journal article Restricted access
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dc.contributor.authorPinto, Miguel
dc.contributor.authorAntelo, Minia
dc.contributor.authorFerreira, Rita
dc.contributor.authorAzevedo, Jacinta
dc.contributor.authorSanto, Irene
dc.contributor.authorBorrego, Maria José
dc.contributor.authorGomes, João Paulo
dc.date.accessioned2018-03-06T15:39:34Z
dc.date.available2018-03-06T15:39:34Z
dc.date.issued2017-03
dc.description.abstractSyphilis is the sexually transmitted disease caused by Treponema pallidum, a pathogen highly adapted to the human host. As a multistage disease, syphilis presents distinct clinical manifestations that pose different implications for diagnosis. Nevertheless, the inherent factors leading to diverse disease progressions are still unknown. We aimed to assess the association between treponemal loads and dissimilar disease outcomes, to better understand syphilis. We retrospectively analyzed 309 DNA samples distinct anatomic sites associated with particular syphilis manifestations. All samples had previously tested positive by a PCR-based diagnostic kit. An absolute quantitative real-time PCR procedure was used to precisely quantify the number of treponemal and human cells to determine T. pallidum loads in each sample. In general, lesion exudates presented the highest T. pallidum loads in contrast with blood-derived samples. Within the latter, a higher dispersion of T. pallidum quantities was observed for secondary syphilis. T. pallidum was detected in substantial amounts in 37 samples of seronegative individuals and in 13 cases considered as syphilis-treated. No association was found between treponemal loads and serological results or HIV status. This study suggests a scenario where syphilis may be characterized by: i) heterogeneous and high treponemal loads in primary syphilis, regardless of the anatomic site, reflecting dissimilar duration of chancres development and resolution; ii) high dispersion of bacterial concentrations in secondary syphilis, potentially suggesting replication capability of T. pallidum while in the bloodstream; and iii) bacterial evasiveness, either to the host immune system or antibiotic treatment, while remaining hidden in privileged niches. This work highlights the importance of using molecular approaches to study uncultivable human pathogens, such as T. pallidum, in the infection process.pt_PT
dc.description.sponsorshipThis work was supported by the Portuguese Foundation for Science and Technology (EXPL/BIA-MIC/0309/2013).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMicrob Pathog. 2017 Mar;104:296-302. doi: 10.1016/j.micpath.2017.01.059. Epub 2017 Feb 2.pt_PT
dc.identifier.doi10.1016/j.micpath.2017.01.059pt_PT
dc.identifier.issn0882-4010 (print)
dc.identifier.issn1096-1208 (online)
dc.identifier.urihttp://hdl.handle.net/10400.18/5214
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier/Academic Presspt_PT
dc.relationNovel approach to "understand" syphilis
dc.relation.publisherversionhttps://linkinghub.elsevier.com/retrieve/pii/S0882-4010(16)30147-4pt_PT
dc.subjectCross-Sectional Studiespt_PT
dc.subjectFemalept_PT
dc.subjectHumanspt_PT
dc.subjectMalept_PT
dc.subjectPortugalpt_PT
dc.subjectReal-Time Polymerase Chain Reactionpt_PT
dc.subjectRetrospective Studiespt_PT
dc.subjectSerologic Testspt_PT
dc.subjectSyphilispt_PT
dc.subjectTreponema pallidumpt_PT
dc.subjectBacterial Loadpt_PT
dc.subjectInfecções Sexualmente Transmissíveispt_PT
dc.titleA retrospective cross-sectional quantitative molecular approach in biological samples from patients with syphilispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNovel approach to "understand" syphilis
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/EXPL%2FBIA-MIC%2F0309%2F2013/PT
oaire.citation.endPage302pt_PT
oaire.citation.startPage296pt_PT
oaire.citation.titleMicrobial Pathogenesispt_PT
oaire.citation.volume104pt_PT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication45d2b04a-2c2a-4464-ae80-e3d4fd449f3c
relation.isProjectOfPublication.latestForDiscovery45d2b04a-2c2a-4464-ae80-e3d4fd449f3c

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