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Investigation of In Vitro Cytotoxicity and Genotoxicity of Novel Cellulose Nanomaterials in two intestinal cell models

2023-09-20Conference object Restricted access
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dc.contributor.authorVital, Nádia
dc.contributor.authorSilva, Maria João
dc.contributor.authorKranendonk, Michel
dc.contributor.authorLouro, Henriqueta
dc.date.accessioned2024-02-15T15:21:19Z
dc.date.available2024-02-15T15:21:19Z
dc.date.issued2023-09-20
dc.descriptionThanks to P. Ferreira, J.Pedrosa (University of Coimbra) and A.F.Lourenço (RAIZ) for the CMNs; to P.Alvito, R.Assunção, M.Silva (Food Safety Department, INSA) for in vitro digestion.pt_PT
dc.description.abstractCellulose nanomaterials (CNMs) have been developed for applications in multiple food-related products, as food additives (e.g. stabilizers or thickeners), non-caloric fiber sources, or substitutes for petroleum-based food packaging materials(1,2). This work aimed to contribute for the safety assessment of two micro/nanofibrillated celluloses (CMF/CNFs), synthetized from industrial Eucalyptus globulus kraft. Based on the European Food Safety Authority nano guidance(3), the in vitro cyto- and genotoxic effects were investigated using the Caco-2 and HT29-MTX-E12 human intestinal cell models. To incorporate the effect of the digestion process on the toxicological outcomes, a harmonized protocol for in vitro simulation of human digestion was used(3). After exposure of cells to digested and undigested CNMs samples (concentrations of 3.1 to 200 μg/mL), the cytotoxicity was evaluated by the MTT and clonogenic assays, and the genotoxicity by the cytokinesis block micronucleus (CBMN; OCDE TG 487(4)) and comet assays. No cytotoxic effects could be attributed to CNMs exposure, with and without digestion, regardless of the cell line used. No chromosomal damage was detected in the two cell lines exposed to each CNMs for 52h, using the micronucleus assay. Conversely, the comet assay revealed the induction of DNA damage in HT29-MTX-E12 cells, after 3h and 24h of exposure to the two CNMs, without significant contribution of oxidative DNA damage. Additionally, in the same cell line, a mild increase in DNA damage, was observed after exposure to the digested CNF comparatively to not digested CNF, after 3h exposure. To our knowledge, this is the first study in which CNMs were evaluated for their genotoxic effects using the CBMN and comet assays in Caco-2 and HT29MTXE12 cell models. Our findings show that cytotoxicity, the endpoint generally used to assess their biocompatibility, is not sufficient to assess their safety to humans. Ongoing studies will contribute to a more comprehensive early-stage assessment of CNMs safety, towards sustainable and innovative application in food technology.pt_PT
dc.description.sponsorshipFunded by FCT/MCTES through 2020.07168.BD (NV), PTDC/ SAUPUB/29481/2017, and ToxOmics (UIDB/00009/2020;UIDP/00009/2020).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/9117
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationToxicological profile of cellulose nanomaterials in human gastrointestinal cells
dc.relationCellular and Molecular Mechanisms of Toxicity of Ingested Nanomaterials
dc.relationCentre for Toxicogenomics and Human Health
dc.relationCentre for Toxicogenomics and Human Health
dc.subjectCellulose Nanomaterialspt_PT
dc.subjectNanomaterialspt_PT
dc.subjectEnvironmental Genotoxicitypt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleInvestigation of In Vitro Cytotoxicity and Genotoxicity of Novel Cellulose Nanomaterials in two intestinal cell modelspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleToxicological profile of cellulose nanomaterials in human gastrointestinal cells
oaire.awardTitleCellular and Molecular Mechanisms of Toxicity of Ingested Nanomaterials
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//2020.07168.BD/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso para Financiamento de Projetos de Investigação Científica e Desenvolvimento Tecnológico em Todos os Domínios Científicos - 2017/PTDC%2FSAU-PUB%2F29481%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00009%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00009%2F2020/PT
oaire.citation.conferencePlaceBraga Portugalpt_PT
oaire.citation.titleConference on Tools and Tests for Safety-by-Design on nanomaterials, 20 september 2023pt_PT
oaire.fundingStreamConcurso para Financiamento de Projetos de Investigação Científica e Desenvolvimento Tecnológico em Todos os Domínios Científicos - 2017
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameVital
person.familyNameSilva
person.familyNameKranendonk
person.familyNameLouro
person.givenNameNádia
person.givenNameMaria Joao
person.givenNameMichel
person.givenNameHenriqueta
person.identifier117538
person.identifier157627
person.identifier.ciencia-id7710-643D-97A3
person.identifier.ciencia-idFC1B-515E-3C03
person.identifier.ciencia-id721D-2BB1-7DB1
person.identifier.orcid0000-0002-3391-0268
person.identifier.orcid0000-0002-6060-0716
person.identifier.orcid0000-0003-1362-0076
person.identifier.orcid0000-0001-9744-7332
person.identifier.ridH-8176-2013
person.identifier.scopus-author-id55944437600
person.identifier.scopus-author-id6603648823
person.identifier.scopus-author-id6507971479
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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