Publicação
Genomic epidemiology and resistome dynamics of species in a Portuguese Open Air Laboratory: the emergence of the FRI-8 carbapenemase
| dc.contributor.author | Teixeira, Pedro | |
| dc.contributor.author | Ramos, Miguel | |
| dc.contributor.author | Rivière, Rani | |
| dc.contributor.author | Azevedo, Mónica | |
| dc.contributor.author | Ferreira, Mário | |
| dc.contributor.author | Cano, Maria Manuela | |
| dc.contributor.author | Vieira, Patrícia | |
| dc.contributor.author | Reis, Lígia | |
| dc.contributor.author | Matias, Rui | |
| dc.contributor.author | Rodrigues, João | |
| dc.contributor.author | Menezes, Carina | |
| dc.contributor.author | Rosado, Tânia | |
| dc.contributor.author | Sequeira, António | |
| dc.contributor.author | Moreira, Olga | |
| dc.contributor.author | Ruppitsch, Werner | |
| dc.contributor.author | Cabal-Rosel, Adriana | |
| dc.contributor.author | Mo, Solveig Sølverød | |
| dc.contributor.author | Dias, Elsa | |
| dc.contributor.author | Woegerbauer, Markus | |
| dc.contributor.author | Caniça, Manuela | |
| dc.contributor.author | Manageiro, Vera | |
| dc.date.accessioned | 2026-02-05T15:50:20Z | |
| dc.date.available | 2026-02-05T15:50:20Z | |
| dc.date.issued | 2025-07-31 | |
| dc.description.abstract | Interconnected reservoirs contribute to the global spread of antimicrobial resistance (AMR), including carbapenem- and colistin-resistant , highlighting the need for a One Health approach. We assessed the genomic epidemiology, diversity and AMR mechanisms of spp. across interconnected human, animal, plant, and environmental reservoirs in a Portuguese Open Air Laboratory. Over a one year monitoring period, samples from 12 different compartments were collected and processed using selective media to isolate spp., which were subjected to antibiotic susceptibility testing, whole-genome sequencing and subsequent analyses to identify AMR determinants, characterize plasmids and phylogenetic relationships. We established a collection of 61 isolates spanning nine species and 32 sequence types, including 16 novel ones, across nine compartments (river water, wastewater, soil, manure, feed, air, farmers, pigs, wild animals), reflecting the diversity and ubiquity of species. Core-genome analysis revealed eight genetic clusters, suggesting clonal transmission across compartments. In total, 29 antibiotic resistance genes were detected across all isolates. Notably, this is the first documentation of -harbouring in European environmental settings and the first to describe , and genes in Portugal. was detected in all isolates ( = 17), located on four different IncFII(Yp) plasmids, and in an isolate, flanked by IS3 family transposases. and the -harbouring isolate were resistant to carbapenems. A gene was identified in an isolate on an IncFII(pECLA) plasmid. These plasmids exhibited high sequence similarity with global counterparts, indicating potential for horizontal gene transfer. Other antimicrobial resistance genes included , , and . Our findings underscore the importance of as vectors for AMR and the critical role of environmental compartments in its dissemination, reinforcing the importance of adopting a One Health approach to fully understand AMR dynamics. | eng |
| dc.description.sponsorship | This work was supported by funding from the European Union’s Horizon 2020 Research and Innovation programme under grant agreement no 773830: One Health European Joint Programme (FED-AMR project), and from Agendas/Alianças mobilizadoras para a Reindustrialização (no. 5, SMARTgNOSTICS project). | |
| dc.identifier.citation | Front Microbiol. 2025 Jul 31:16:1593872. doi: 10.3389/fmicb.2025.1593872. eCollection 2025 | |
| dc.identifier.doi | 10.3389/fmicb.2025.1593872 | |
| dc.identifier.eissn | 1664-302X | |
| dc.identifier.pmid | 40822392 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/10820 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | Frontiers Media | |
| dc.relation | Promoting One Health in Europe through joint actions on foodborne zoonoses, antimicrobial resistance and emerging microbiological hazards. | |
| dc.relation.hasversion | https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2025.1593872/full | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Enterobacter vonholyi | |
| dc.subject | FRI-8 and IMI-6 carbapenemases | |
| dc.subject | MCR-10 | |
| dc.subject | carbapenem-resistant Enterobacterales | |
| dc.subject | colistin-resistance | |
| dc.subject | one health | |
| dc.subject | Resistência aos Antimicrobianos | |
| dc.title | Genomic epidemiology and resistome dynamics of species in a Portuguese Open Air Laboratory: the emergence of the FRI-8 carbapenemase | eng |
| dc.type | journal article | |
| dcterms.references | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1593872/full#supplementary-material | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Promoting One Health in Europe through joint actions on foodborne zoonoses, antimicrobial resistance and emerging microbiological hazards. | |
| oaire.awardURI | info:eu-repo/grantAgreement/EC/H2020/773830/EU | |
| oaire.citation.startPage | 1593872 | |
| oaire.citation.title | Frontiers in Microbiology | |
| oaire.citation.volume | 16 | |
| oaire.fundingStream | H2020 | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |
| project.funder.identifier | http://doi.org/10.13039/501100008530 | |
| project.funder.name | European Commission | |
| relation.isProjectOfPublication | 94d118fb-33ce-49fa-b1ed-d5bddf63581d | |
| relation.isProjectOfPublication.latestForDiscovery | 94d118fb-33ce-49fa-b1ed-d5bddf63581d |