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New insights into resistance to colistin and third-generation cephalosporins of Escherichia coli in poultry, Portugal: Novel blaCTX-M-166 and blaESAC genes

2017-12-18Journal article Restricted access
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dc.contributor.authorManageiro, Vera
dc.contributor.authorClemente, Lurdes
dc.contributor.authorGraça, Rafael
dc.contributor.authorCorreia, Ivone
dc.contributor.authorAlbuquerque, Teresa
dc.contributor.authorFerreira, Eugénia
dc.contributor.authorCaniça, Manuela
dc.date.accessioned2018-03-07T18:38:28Z
dc.date.available2018-03-07T18:38:28Z
dc.date.issued2017-12-18
dc.description.abstractThe increasing incidence of intestinal colonization with extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae and Gram negative organisms that has been observed in food animals such as poultry, cattle and pigs, are suggestive that animals, food and environment are potential sources of ESBL-producing bacteria. Hence, the aim of this study was to characterized commensal E. coli obtained from healthy broiler and turkey flocks at slaughter for the presence of penicillinases-, ESBL-, extended-spectrum AmpC (ESAC)-, plasmid-mediated quinolone resistance- and MCR-encoding genes. Study of clonal relatedness showed genetic diversity among CTX-M-type, SHV-12 and TEM-52 producing isolates with human isolates of the same type, was also assessed. We detected that eleven (5.4%, 11/202) and forty-five (2.2%, 45/185) E. coli isolates from broilers and turkeys, respectively, carried blaESBLor blaESACgenes and two isolates from turkeys carried mcr-1 gene. A new variant blaCTX-M-166was reported in a multidrug resistant isolate from a broiler flock. Overall, we detected a diversity of resistance mechanisms among E. coli from food-producing animals, all of them with high importance at a public health level.pt_PT
dc.description.abstractHighlights: Detection of blaESBL, blaESAC and the recently described mcr-1 gene; New variant blaCTX-M-166 reported in a MDR isolate from a broiler flock; WGS approach revealed a CTX-M-166-harboring O6:H16 ST48-fimH34 E. coli; ESBL-producing E. coli in the poultry population was not due to one specific clone.pt_PT
dc.description.sponsorshipThe authors thank Fundação para a Ciência e a Tecnologia (FCT) for project grant PEst-OE/AGR/UI0211/2011-2014, Strategic Project UI211-2011-2014. V. Manageiro was supported by FCT fellowship (grant SFRH/BPD/77486/2011), financed by the European Social Funds (COMPETE-FEDER) and national funds of the Portuguese Ministry of Education and Science (POPH-QREN)pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationInt J Food Microbiol. 2017 Dec 18;263:67-73. doi: 10.1016/j.ijfoodmicro.2017.10.007. Epub 2017 Oct 4.pt_PT
dc.identifier.doi10.1016/j.ijfoodmicro.2017.10.007pt_PT
dc.identifier.issn0168-1605
dc.identifier.urihttp://hdl.handle.net/10400.18/5352
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationEVOLUTIONARY POTENTIAL OF OXYIMINO-BETA-LACTAM RESISTANCE DETERMINANTS IN ANIMALS AND IDENTIFICATION OF NEW ENZYME-INHIBITORS FOR VETERINARY USE
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0168160517304166?via%3Dihubpt_PT
dc.subjectAnimalspt_PT
dc.subjectAnti-Bacterial Agentspt_PT
dc.subjectCephalosporinspt_PT
dc.subjectChickenspt_PT
dc.subjectColistinpt_PT
dc.subjectEscherichia colipt_PT
dc.subjectEscherichia coli Infectionspt_PT
dc.subjectEscherichia coli Proteinspt_PT
dc.subjectFood Contaminationpt_PT
dc.subjectPlasmidspt_PT
dc.subjectPortugalpt_PT
dc.subjectPoultry Diseasespt_PT
dc.subjectTurkeyspt_PT
dc.subjectbeta-Lactamasespt_PT
dc.subjectResistência aos Antimicrobianospt_PT
dc.titleNew insights into resistance to colistin and third-generation cephalosporins of Escherichia coli in poultry, Portugal: Novel blaCTX-M-166 and blaESAC genespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleEVOLUTIONARY POTENTIAL OF OXYIMINO-BETA-LACTAM RESISTANCE DETERMINANTS IN ANIMALS AND IDENTIFICATION OF NEW ENZYME-INHIBITORS FOR VETERINARY USE
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/PEst-OE%2FAGR%2FUI0211%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F77486%2F2011/PT
oaire.citation.endPage73pt_PT
oaire.citation.startPage67pt_PT
oaire.citation.titleInternational Journal of Food Microbiologypt_PT
oaire.citation.volume263pt_PT
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationd3e4188b-642e-4ad7-9a4d-aeea65a89449
relation.isProjectOfPublication3b63e7d9-d684-42ae-aff9-36815c617501
relation.isProjectOfPublication.latestForDiscoveryd3e4188b-642e-4ad7-9a4d-aeea65a89449

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