Repository logo
 
Publication

Epidemiology and genetic variability of respiratory syncytial virus in Portugal, 2014-2018

2019-10-10Journal article Restricted access
Simple item page
dc.contributor.authorSáez-López, Emma
dc.contributor.authorCristóvão, Paula
dc.contributor.authorCosta, Inês
dc.contributor.authorPechirra, Pedro
dc.contributor.authorConde, Patrícia
dc.contributor.authorGuiomar, Raquel
dc.contributor.authorPeres, Maria João
dc.contributor.authorViseu, Regina
dc.contributor.authorLopes, Paulo
dc.contributor.authorSoares, Vânia
dc.contributor.authorVale, Fátima
dc.contributor.authorFonseca, Patricia
dc.contributor.authorFreitas, Ludivina
dc.contributor.authorAlves, Jose
dc.contributor.authorPessanha, Maria Ana
dc.contributor.authorToscano, Cristina
dc.contributor.authorMota-Vieira, Luísa
dc.contributor.authorVeloso, Rita Cabral
dc.contributor.authorCôrte-Real, Rita
dc.contributor.authorBranquinho, Paula
dc.contributor.authorPereira‑Vaz, João
dc.contributor.authorRodrigues, Fernando
dc.contributor.authorCunha, Mário
dc.contributor.authorMartins, Luís
dc.contributor.authorMota, Paula
dc.contributor.authorCouto, Ana Rita
dc.contributor.authorBruges-Armas, Jácome
dc.contributor.authorAlmeida, Sofia
dc.contributor.authorRodrigues, Débora
dc.contributor.authorPortuguese Laboratory Network for the Diagnosis of Influenza Infection
dc.date.accessioned2020-05-05T23:06:26Z
dc.date.available2020-05-05T23:06:26Z
dc.date.issued2019-10-10
dc.descriptionFree PMC article: https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/31707201/
dc.description.abstractIntroduction: Respiratory syncytial virus (RSV) is associated with substantial morbidity and mortality since it is a predominant viral agent causing respiratory tract infections in infants, young children and the elderly. Considering the availability of the RSV vaccines in the coming years, molecular understanding in RSV is necessary. Objective: The objective of the present study was to describe RSV epidemiology and genotype variability in Portugal during the 2014/15-2017/18 period. Material and methods: Epidemiological data and RSV-positive samples from patients with a respiratory infection were collected through the non-sentinel and sentinel influenza surveillance system (ISS). RSV detection, subtyping in A and B, and sequencing of the second hypervariable region (HVR2) of G gene were performed by molecular methods. Phylogenetic trees were generated using the Neighbor-Joining method and p-distance model on MEGA 7.0. Results: RSV prevalence varied between the sentinel (2.5%, 97/3891) and the non-sentinel ISS (20.7%, 3138/16779), being higher (P < 0.0001) among children aged <5 years. Bronchiolitis (62.9%, 183/291) and influenza-like illness (24.6%, 14/57) were associated (P < 0.0001) with RSV laboratory confirmation among children aged <6 months and adults ≥65 years, respectively. The HVR2 was sequenced for 562 samples. RSV-A (46.4%, 261/562) and RSV-B (53.6%, 301/562) strains clustered mainly to ON1 (89.2%, 233/261) and BA9 (92%, 277/301) genotypes, respectively, although NA1 and BA10 were also present until 2015/2016. Conclusion: The sequence and phylogenetic analysis reflected the relatively high diversity of Portuguese RSV strains. BA9 and ON1 genotypes, which have been circulating in Portugal since 2010/2011 and 2011/2012 respectively, predominated during the whole study period.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJ Clin Virol. 2019 Dec;121:104200. doi: 10.1016/j.jcv.2019.104200. Epub 2019 Oct 10pt_PT
dc.identifier.doi10.1016/j.jcv.2019.104200pt_PT
dc.identifier.issn1386-6532
dc.identifier.urihttp://hdl.handle.net/10400.18/6599
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S1386653219302185?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectRespiratory Syncytial Viruspt_PT
dc.subjectBA9 Genotypept_PT
dc.subjectGenetic Diversitypt_PT
dc.subjectMolecular Epidemiologypt_PT
dc.subjectON1 Genotypept_PT
dc.subjectPortugalpt_PT
dc.subjectInfecções Respiratóriaspt_PT
dc.titleEpidemiology and genetic variability of respiratory syncytial virus in Portugal, 2014-2018pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage104200pt_PT
oaire.citation.titleJournal of Clinical Virologypt_PT
oaire.citation.volume121pt_PT
rcaap.embargofctDe acordo com política editorial da revista.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Epidemiology and genetic variability.pdf
Size:
3.14 MB
Format:
Adobe Portable Document Format
Download

Collections

DDI - Artigos em revistas internacionais