Publication
Genetic diversity and clonal evolution of carbapenem-resistant Acinetobacter baumannii isolates from Portugal and the dissemination of ST118
| dc.contributor.author | Manageiro, Vera | |
| dc.contributor.author | Jones-Dias, Daniela | |
| dc.contributor.author | Ferreira, Eugénia | |
| dc.contributor.author | Louro, Deolinda | |
| dc.contributor.author | Antimicrobial Resistance Surveillance Program in Portugal (ARSIP) | |
| dc.contributor.author | Caniça, Manuela | |
| dc.date.accessioned | 2013-02-14T16:29:59Z | |
| dc.date.available | 2013-02-14T16:29:59Z | |
| dc.date.issued | 2012 | |
| dc.description.abstract | In this study, 116 multidrug-resistant Acinetobacter baumannii (MDR-Ab) isolates recovered in various regions of Portugal were studied. All isolates were non-susceptible to tigecycline; one isolate was also non-susceptible to colistin, making it a step closer to pandrug resistance. Among 72 isolates tested by PFGE, 98.6% carried blaOXA-66, 1.4% blaOXA-104, 77.8% blaOXA-23, 23.6% blaOXA-24, 18.1% blaTEM-1 and 1.4% blaCTX-M-15-like genes. No OXA-58 or metallo-β-lactamase-encoding genes were detected. ISAba1 was found in 58/72 isolates (80.6%). Among these, ISAba1 was found upstream of blaOXA-51-like in 54 isolates. All but two of these isolates also carried ISAba1–blaOXA-23, highlighting the coexistence of ISAba1–blaOXA-51-like and ISAba1–blaOXA-23 genetic platforms, emphasising the importance of mobile genetic elements in the dissemination of carbapenem-hydrolysing class D β-lactamase genes. Tn2006-like and Tn2008-like, found within ST92 and ST118, may reflect either multiple genetic structures in the origin of blaOXA-23 acquisition or interclonal complex evolution. These results indicate that there may exist different genetic origins for carbapenem resistance among MDR-Ab isolates. Six PFGE profiles were associated with three major sequence types, with ST118 (OXA-23- or OXA-24-producer) being widely disseminated since 2009. ST98 (described so far as endemic in Portugal) and ST92 (which co-existed with ST98 before 2009) appeared to have been gradually replaced by ST118. The new ST188 (OXA-104-producer) was detected for the first time in this country. Identification of an extensively drug-resistant ST118 and carbapenem-resistant ST92, ST98 and ST118 isolates, both in community and healthcare facilities, demonstrates the menace of A. baumannii-associated infections. | por |
| dc.description.sponsorship | VM has received research funding from grant SFRH/BD/32578/2006 from the Fundação para a Ciência e a Tecnologia (Lisbon, Portugal). DJ-D has received research funding from grant BRJ/02/DG/2009 from the National Institute of Health Dr Ricardo Jorge (Lisbon, Portugal). | por |
| dc.identifier.citation | Int J Antimicrob Agents. 2012 Nov;40(5):398-403. Epub 2012 Aug 11. | por |
| dc.identifier.issn | doi: 10.1016/j.ijantimicag.2012.06.013 | |
| dc.identifier.other | 0924-8579 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/1369 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Elsevier/ International Society of Chemotherapy | por |
| dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S0924857912002683 | por |
| dc.subject | OXA-23 | por |
| dc.subject | OXA-24 | por |
| dc.subject | ST118 | por |
| dc.subject | Switching | por |
| dc.subject | ISAba1 | por |
| dc.subject | XDR | por |
| dc.subject | Resistência aos Antimicrobianos | por |
| dc.title | Genetic diversity and clonal evolution of carbapenem-resistant Acinetobacter baumannii isolates from Portugal and the dissemination of ST118 | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 403 | por |
| oaire.citation.startPage | 398 | por |
| oaire.citation.title | International Journal of Antimicrobial Agents | por |
| oaire.citation.volume | 40(5) | por |
| rcaap.rights | restrictedAccess | por |
| rcaap.type | article | por |