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Development and In Vitro Validation of Antibacterial Paints Containing Chloroxylenol and Terpineol

2022-06-21Journal article Open access
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dc.contributor.authorQuerido, Micaela Machado
dc.contributor.authorPaulo, Ivo
dc.contributor.authorHariharakrishnan, Sriram
dc.contributor.authorRocha, Daniel
dc.contributor.authorBarbosa, Nuno
dc.contributor.authorGonçalves, Diogo
dc.contributor.authordos Santos, Rui Galhano
dc.contributor.authorBordado, João Moura
dc.contributor.authorTeixeira, João Paulo
dc.contributor.authorPereira, Cristiana Costa
dc.date.accessioned2023-01-10T15:46:50Z
dc.date.available2023-01-10T15:46:50Z
dc.date.issued2022-06-21
dc.descriptionThis article belongs to the Section Toxicology.pt_PT
dc.description.abstractThe establishment of self-disinfecting surfaces is an important method to avoid surface contamination. Recently, paints with antimicrobial properties have been developed to be applied on different surfaces, avoiding contamination with pathogens. In this work, self-disinfecting paints containing Chloroxylenol (CLX), Terpineol (TRP), and a mixture of both substances were developed. The goal was to evaluate and validate these paints using international standards for eventual commercialization and application in scenarios where surface contamination represents a problem. The paints were challenged with five different bacteria, Gram-positive and Gram- negative, before and after a scrub resistance test, where the long-term efficacy of the paints was evaluated. The antibacterial activity assessment was performed following ISO 22196 and JIS Z2801. In general, the paints showed very promising results, demonstrating their antibacterial activity, before and after scrub resistance test. The paint incorporating the mixture of CLX and TRP (CLX+TRP) stood out by revealing consistent results of antibacterial activity both before and after the scrub resistance test for most of the tested bacteria. The cytotoxicity of the developed paints was assessed in vitro by performing tests by direct contact with a human skin cell line, HaCaT, and testes on extracts with HaCaT and a pulmonary cell line, A549. The methodologies for cytotoxicity assessment were developed based in ISO 10993. For genotoxicity assessment, alkaline comet assay was conducted on both cell lines. The cytotoxicity assessment revealed promising results with the paints, demonstrating values of cellular viability above 70% and values of lactate dehydrogenase (LDH) leakage below 30%. The genotoxic assessment also revealed acceptable values of primary DNA damage for the developed antibacterial paints. In general, the selected methodologies presented good potential to be applied in the validation of both efficacy and safety of the antimicrobial paints, aiming to be applied in real scenarios.pt_PT
dc.description.sponsorshipThis research was funded by Programa Operacional Competitividade e Internacionaliza ção—COMPETE2020/FEDER, towards the project B-Safecoat (POCI-01-0247-FEDER-017875), and by FCT (Fundação para a Ciência e Tecnologia) through the PhD grant awarded to M.M.Q. (SFRH/BD/130203/2017)pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationToxics. 2022 Jun 21;10(7):343. doi: 10.3390/toxics10070343pt_PT
dc.identifier.doi10.3390/ toxics10070343pt_PT
dc.identifier.issn2305-6304
dc.identifier.urihttp://hdl.handle.net/10400.18/8418
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationDesinFAST-Self-Disinfecting Paint for Infection Control in Public Spaces
dc.relation.publisherversionhttps://www.mdpi.com/2305-6304/10/7/343/htmlpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectPaintpt_PT
dc.subjectAntibacterialpt_PT
dc.subjectChloroxylenolpt_PT
dc.subjectTerpineolpt_PT
dc.subjectCytotoxicitypt_PT
dc.subjectToxicologiapt_PT
dc.titleDevelopment and In Vitro Validation of Antibacterial Paints Containing Chloroxylenol and Terpineolpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDesinFAST-Self-Disinfecting Paint for Infection Control in Public Spaces
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F130203%2F2017/PT
oaire.citation.issue7pt_PT
oaire.citation.startPage343pt_PT
oaire.citation.titleToxicspt_PT
oaire.citation.volume10pt_PT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication8c1a52a8-15d9-4bd0-b94c-8320cbef5a1e
relation.isProjectOfPublication.latestForDiscovery8c1a52a8-15d9-4bd0-b94c-8320cbef5a1e

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