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A DNA barcode reference library of Portuguese mosquitoes

2021-08-16Journal article Open access
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dc.contributor.authorMadeira, S.
dc.contributor.authorDuarte, A.
dc.contributor.authorBoinas, F.
dc.contributor.authorOsório, H.C.
dc.date.accessioned2022-07-12T14:40:12Z
dc.date.available2022-07-12T14:40:12Z
dc.date.issued2021-08-16
dc.description.abstractMosquitoes are important biological vectors of pathogens and species identification in all life stages is the first step for effective monitoring and control of mosquito-borne diseases. Molecular methods for species identification have been developed over the last years to overcome the limitations of the taxonomic identification based on morphology. DNA barcoding, using a fragment of the mitochondrial cytochrome oxidase I (COI) gene, can be used for species identification but a reliable and comprehensive reference database of verified sequences is required. In this study, we aimed to generate a DNA barcode reference library for the identification of mosquito species from Portuguese mosquito fauna, including most relevant vector species. Mosquitoes captured under the National Vector Surveillance Program (REVIVE) were processed for DNA extraction, COI gene fragment amplification and sequencing. Nighty-eight barcode sequences were obtained, representing 26 species and 6 genera. Sequences were submitted to GenBank and BOLD and were used for validation of phenetic classification. Barcode Index Number (BIN) assignment and Automatic Barcode Gap Discovery (ABGD) were used and clustered COI sequences into twenty-five molecular operational taxonomic units (MOTUs). This is the first comprehensive study that combines morphological and molecular identification of most mosquito species present in Portugal aiming to offer a reliable framework for mosquito species identification.pt_PT
dc.description.sponsorshipThe research was financially supported by FCT - Fundação para a Ciência e a Tecnologia, I.P., through the project grant UIDB/00276/2020 (CIISA - Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Lisboa, Portugal). SM was supported by a FCT PhD fellowship SFRH/BD/117431/2016pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationZoonoses Public Health. 2021 Dec;68(8):926-936. doi: 10.1111/zph.12885. Epub 2021 Aug 16pt_PT
dc.identifier.doi10.1111/zph.12885pt_PT
dc.identifier.issn1863-1959
dc.identifier.urihttp://hdl.handle.net/10400.18/8232
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationCentre for Interdisciplinary Research in Animal Health
dc.relationEmergent arboviruses of ruminants and equids – a study of the potential insect vectors in Portugal
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/10.1111/zph.12885pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectCulicidaept_PT
dc.subjectCytochrome Oxidase I (COI)pt_PT
dc.subjectDNA Barcodingpt_PT
dc.subjectMosquito Surveillancept_PT
dc.subjectPortugalpt_PT
dc.subjectSpecies Identificationpt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.titleA DNA barcode reference library of Portuguese mosquitoespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Interdisciplinary Research in Animal Health
oaire.awardTitleEmergent arboviruses of ruminants and equids – a study of the potential insect vectors in Portugal
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00276%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F117431%2F2016/PT
oaire.citation.endPage936pt_PT
oaire.citation.issue68pt_PT
oaire.citation.startPage926pt_PT
oaire.citation.titleZoonoses Public Healthpt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication17f6ecf9-26db-4596-b594-6b8e97bb3f5c
relation.isProjectOfPublicationbb8077be-d69e-4887-9f6c-f91aea9148f2
relation.isProjectOfPublication.latestForDiscovery17f6ecf9-26db-4596-b594-6b8e97bb3f5c

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