Publication
Second worldwide proficiency study on variable number of tandem repeats typing of Mycobacterium tuberculosis complex
| dc.contributor.author | de Beer, J.L. | |
| dc.contributor.author | Ködmön, C. | |
| dc.contributor.author | van Ingen, J. | |
| dc.contributor.author | Supply, P. | |
| dc.contributor.author | van Soolingen, D. | |
| dc.contributor.author | Global Network for the Molecular Surveillance of Tuberculosis 2010 | |
| dc.date.accessioned | 2016-10-06T15:35:59Z | |
| dc.date.available | 2016-10-06T15:35:59Z | |
| dc.date.issued | 2014-05 | |
| dc.description | Global Network for the Molecular Surveillance of Tuberculosis 2010: A. Miranda (Tuberculosis Laboratory of the National Institute of Health, Porto, Portugal) | pt_PT |
| dc.description.abstract | BACKGROUND: The quality of variable number of tandem repeats (VNTR) typing of Mycobacterium tuberculosis was first investigated in 2009 in 37 laboratories worldwide. The results revealed an inter- and intra-laboratory reproducibility of respectively 60% and 72%. These data spurred an improvement in laboratory-specific assays and global standardisation of VNTR typing. OBJECTIVE: To measure the effects of the technical improvements and increased standardisation, a test panel consisting of 30 M. tuberculosis complex DNA samples was distributed for VNTR typing in 41 participating laboratories from 36 countries. RESULTS: The inter- and intra-laboratory reproducibil- ity increased overall to respectively 78% and 88%. The 33 laboratories that participated in both the first and second proficiency studies improved their inter- and intra-laboratory reproducibility from 62% and 72% to respectively 79% and 88%. The largest improvement in reproducibility was detected in 10 laboratories that use an in-house polymerase chain reaction technique and perform amplicon sizing using gel electrophoresis. Detailed error analysis revealed a reduction in the number of systematic errors, sample exchange events and non-amplifiable loci. CONCLUSION: This second worldwide proficiency study indicates a substantial increase in the reproduc- ibility of VNTR typing of M. tuberculosis. This will contribute to a more meaningful interpretation of molecular epidemiological and phylogenetic studies on the M. tuberculosis complex. | pt_PT |
| dc.identifier.citation | Int J Tuberc Lung Dis. 2014 May;18(5):594-600. doi: 10.5588/ijtld.13.0531 | pt_PT |
| dc.identifier.doi | 10.5588/ijtld.13.0531 | pt_PT |
| dc.identifier.issn | 1027-3719 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/4043 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | International Union Against Tuberculosis and Lung Disease | pt_PT |
| dc.relation | Multidrug-resistant Tuberculosis Molecular Surveillance Project. The project is coordinated by the ECDC and the implementation outsourced to the National Institute for Public Health and the Environment under service contract ECDC/08/019 (February 2009–February 2012) | pt_PT |
| dc.relation.publisherversion | http://www.ingentaconnect.com/content/iuatld/ijtld/2014/00000018/00000005/art00018 | pt_PT |
| dc.subject | VNTR | pt_PT |
| dc.subject | MLVA | pt_PT |
| dc.subject | Tuberculosis | pt_PT |
| dc.subject | Reproducibility | pt_PT |
| dc.subject | Proficiency | pt_PT |
| dc.subject | Quality Control | pt_PT |
| dc.subject | Tandem Repeats | pt_PT |
| dc.subject | Infecções Respiratórias | |
| dc.title | Second worldwide proficiency study on variable number of tandem repeats typing of Mycobacterium tuberculosis complex | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 600 | pt_PT |
| oaire.citation.startPage | 594 | pt_PT |
| oaire.citation.title | International Journal of Tuberculosis and Lung Disease | pt_PT |
| oaire.citation.volume | 18(5) | pt_PT |
| rcaap.rights | embargoedAccess | pt_PT |
| rcaap.type | article | pt_PT |