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Profiling the erythrocyte membrane proteome isolated from patients diagnosed with chronic obstructive pulmonary disease

2012Journal article Restricted access
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dc.contributor.authorAlexandre, Bruno
dc.contributor.authorCharro, Nuno
dc.contributor.authorBlonder, Yosip
dc.contributor.authorLopes, Carlos
dc.contributor.authorAlmeida, Antonio Bugalho
dc.contributor.authorVeenstra, Timothy
dc.contributor.authorPenque, Deborah
dc.contributor.authorAzevedo, Pilar
dc.contributor.authorChan, K.C.
dc.contributor.authorPrieto, D.A.
dc.contributor.authorIssaq, H.
dc.date.accessioned2012-05-31T14:37:38Z
dc.date.available2012-05-31T14:37:38Z
dc.date.issued2012
dc.description.abstractStructural and metabolic alterations in erythrocytes play an important role in the pathophysiology of Chronic Obstructive Pulmonary Disease (COPD). Whether these dysfunctions are related to the modulation of erythrocyte membrane proteins in patients diagnosed with COPD remains to be determined. Herein, a comparative proteomic profiling of the erythrocyte membrane fraction isolated from peripheral blood of smokers diagnosed with COPD and smokers with no COPD was performed using differential 16O/18O stable isotope labeling. A total of 219 proteins were quantified as being significantly differentially expressed within the erythrocyte membrane proteomes of smokers with COPD and healthy smokers. Functional pathway analysis showed that the most enriched biofunctions were related to cell-to-cell signaling and interaction, hematological system development, immune response, oxidative stress and cytoskeleton. Chorein (VPS13A), a cytoskeleton related protein whose defects had been associated with the presence of cell membrane deformation of circulating erythrocytes was found to be down-regulated in the membrane fraction of erythrocytes obtained from COPD patients. Methemoglobin reductase (CYB5R3) was also found to be underexpressed in these cells, suggesting that COPD patients may be at higher risk for developing methemoglobinemia. This article is part of a Special Issue entitled: “Integrated omics— Functional applications to blood and blood therapeutics”.por
dc.identifier.citationJ Proteomics. 2012 Apr 17. [Epub ahead of print]por
dc.identifier.issn1874-3919
dc.identifier.urihttp://hdl.handle.net/10400.18/869
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S187439191200214Xpor
dc.subjectProteomicspor
dc.subjectDPOCpor
dc.subjectRed Blood Cellspor
dc.subjectGenómica Funcionalpor
dc.titleProfiling the erythrocyte membrane proteome isolated from patients diagnosed with chronic obstructive pulmonary diseasepor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleJournal of Proteomicspor
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor

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