Publication
Plasmid-Mediated Colistin Resistance (mcr-1) in Escherichia coli from Non-Imported Fresh Vegetables for Human Consumption in Portugal
| dc.contributor.author | Manageiro, Vera | |
| dc.contributor.author | Jones-Dias, Daniela | |
| dc.contributor.author | Ferreira, Eugénia | |
| dc.contributor.author | Caniça, Manuela | |
| dc.date.accessioned | 2021-03-04T15:20:22Z | |
| dc.date.available | 2021-03-04T15:20:22Z | |
| dc.date.issued | 2020-03-18 | |
| dc.description | Brief Report | pt_PT |
| dc.description.abstract | In this study, we report the presence of the plasmid-mediated colistin resistance (PMCR)-encoding gene mcr-1 in an Escherichiacoli isolate, INSali25, recovered from lettuce produced and marketed in Portugal. Colistin MIC from the vegetable E. coli isolate-determined by microdilution broth method according to EUCAST guidelines-revealed a non-wild-type phenotype of colistin (MIC 16 mg/L). To understand the genetic background of E. coli INSali25, we performed whole genome sequencing. Plasmid sequencing was also performed after plasmid DNA extraction from the transconjugant TcINSali25 (mcr-1). Directed bioinformatics analysis identified the mcr-1 gene in a 39,998 bp length contig, with an upstream region including the antibiotic resistance gene blaTEM-1 in a partial transposon Tn2, truncated by the insertion sequence IS26 and showing >99% identity with previously described mcr-1-harboring IncHI2 plasmids. Further in silico analysis showed the presence of additional genes conferring resistance to β-lactams (blaTEM-1), aminoglycosides (aadA1, aph(4)-Ia, aph(6)-Id, aac(3)-Iv), macrolides (mdf(A)-type), phenicol (floR-type), tetracycline (tetA), and sulphonamides (sul2). INSali25 isolate belonged to the ST1716 lineage and showed the fimH54 and fumC27 alleles. Lettuce is a vegetable that is commonly consumed fresh and not subjected to any cooking process, which may amplify human food safety risks. Moreover, the occurrence of plasmid-mediated colistin resistance in a sample that was not imported and was acquired in a large retail store reinforces the widespread distribution of mcr-1. | pt_PT |
| dc.description.sponsorship | The work was supported by UIDB/00211/2020 with funding from FCT/MCTES through national funds | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Microorganisms. 2020 Mar 18;8(3):429. doi: 10.3390/microorganisms8030429. | pt_PT |
| dc.identifier.doi | 10.3390/microorganisms8030429 | pt_PT |
| dc.identifier.issn | 2076-2607 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/7313 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | MDPI | pt_PT |
| dc.relation | Center for the Study of Animal Science | |
| dc.relation.publisherversion | https://www.mdpi.com/2076-2607/8/3/429 | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Antibiotic Resistance | pt_PT |
| dc.subject | Food | pt_PT |
| dc.subject | mcr-1 | pt_PT |
| dc.subject | Vegetables | pt_PT |
| dc.subject | Colistin resistance | pt_PT |
| dc.subject | Resistência aos Antimicrobianos | pt_PT |
| dc.title | Plasmid-Mediated Colistin Resistance (mcr-1) in Escherichia coli from Non-Imported Fresh Vegetables for Human Consumption in Portugal | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Center for the Study of Animal Science | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00211%2F2020/PT | |
| oaire.citation.issue | 3 | pt_PT |
| oaire.citation.startPage | 429 | pt_PT |
| oaire.citation.title | Microorganisms | pt_PT |
| oaire.citation.volume | 8 | pt_PT |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.embargofct | Acesso de acordo com política editorial da revista. | pt_PT |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isProjectOfPublication | 69b75eb9-6f25-4ad8-98db-6cc7e9bcdcc7 | |
| relation.isProjectOfPublication.latestForDiscovery | 69b75eb9-6f25-4ad8-98db-6cc7e9bcdcc7 |