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Bacterial Diversity and Antibiotic Susceptibility of Sparus aurata from Aquaculture

2020-09-02Journal article Open access
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dc.contributor.authorSalgueiro, Vanessa
dc.contributor.authorManageiro, Vera
dc.contributor.authorBandarra, Narcisa M.
dc.contributor.authorReis, Lígia
dc.contributor.authorFerreira, Eugénia
dc.contributor.authorCaniça, Manuela
dc.date.accessioned2021-03-31T16:30:12Z
dc.date.available2021-03-31T16:30:12Z
dc.date.issued2020-09-02
dc.description.abstractIn a world where the population continues to increase and the volume of fishing catches stagnates or even falls, the aquaculture sector has great growth potential. This study aimed to contribute to the depth of knowledge of the diversity of bacterial species found in Sparus aurata collected from a fish farm and to understand which profiles of diminished susceptibility to antibiotics would be found in these bacteria that might be disseminated in the environment. One hundred thirty-six bacterial strains were recovered from the S. aurata samples. These strains belonged to Bacillaceae, Bacillales Family XII. Incertae Sedis, Comamonadaceae, Enterobacteriaceae, Enterococcaceae, Erwiniaceae, Micrococcaceae, Pseudomonadaceae and Staphylococcaceae families. Enterobacter sp. was more frequently found in gills, intestine and skin groups than in muscle groups (p ≤ 0.01). Antibiotic susceptibility tests found that non-susceptibility to phenicols was significantly higher in gills, intestine and skin samples (45%) than in muscle samples (24%) (p ≤ 0.01) and was the most frequently found non-susceptibility in both groups of samples. The group of Enterobacteriaceae from muscles presented less decreased susceptibility to florfenicol (44%) than in the group of gills, intestine and skin samples (76%). We found decreased susceptibilities to β-lactams and glycopeptides in the Bacillaceae family, to quinolones and mupirocin in the Staphylococcaceae family, and mostly to β-lactams, phenicols and quinolones in the Enterobacteriaceae and Pseudomonadaceae families. Seven Enterobacter spp. and five Pseudomonas spp. strains showed non-susceptibility to ertapenem and meropenem, respectively, which is of concern because they are antibiotics used as a last resort in serious clinical infections. To our knowledge, this is the first description of species Exiguobacterium acetylicum, Klebsiella michiganensis, Lelliottia sp. and Pantoea vagans associated with S. aurata (excluding cases where these bacteria are used as probiotics) and of plasmid-mediated quinolone resistance qnrB19-producing Leclercia adecarboxylata strain. The non-synonymous G385T and C402A mutations at parC gene (within quinolone resistance-determining regions) were also identified in a Klebsiella pneumoniae, revealing decreased susceptibility to ciprofloxacin. In this study, we found not only bacteria from the natural microbiota of fish but also pathogenic bacteria associated with fish and humans. Several antibiotics for which decreased susceptibility was found here are integrated into the World Health Organization list of "critically important antimicrobials" and "highly important antimicrobials" for human medicine.pt_PT
dc.description.sponsorshipVanessa Salgueiro has her Ph.D. fellowship granted by FCT (Fundação para a Ciência e a Tecnologia) with the reference SFRH/BD/133100/2017 co-financed by European Social Fund and the Operational Program for Human Capital (POCH), Portugal.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMicroorganisms. 2020 Sep 2;8(9):1343. doi: 10.3390/microorganisms8091343.pt_PT
dc.identifier.doi10.3390/microorganisms8091343pt_PT
dc.identifier.issn2076-2607
dc.identifier.urihttp://hdl.handle.net/10400.18/7615
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationDecrypting the diversity of microbiome in aquaculture
dc.relation.publisherversionhttps://www.mdpi.com/2076-2607/8/9/1343pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectOne Healthpt_PT
dc.subjectSparus auratapt_PT
dc.subjectAntibiotic Resistancept_PT
dc.subjectAquaculturept_PT
dc.subjectqnrB19pt_PT
dc.subjectResistência aos Antimicrobianospt_PT
dc.titleBacterial Diversity and Antibiotic Susceptibility of Sparus aurata from Aquaculturept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDecrypting the diversity of microbiome in aquaculture
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F133100%2F2017/PT
oaire.citation.issue9pt_PT
oaire.citation.startPage1343pt_PT
oaire.citation.titleMicroorganismspt_PT
oaire.citation.volume8pt_PT
oaire.fundingStreamOE
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationb16cfc8b-bb9b-43b9-9b11-2c49a0856050
relation.isProjectOfPublication.latestForDiscoveryb16cfc8b-bb9b-43b9-9b11-2c49a0856050

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