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The expression of Helicobacter pylori tfs plasticity zone cluster is regulated by pH and adherence, and its composition is associated with differential gastric IL-8 secretion

2017-08Journal article Restricted access
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dc.contributor.authorSilva, Bruno
dc.contributor.authorNunes, Alexandra
dc.contributor.authorVale, Filipa F
dc.contributor.authorRocha, Raquel
dc.contributor.authorGomes, João Paulo
dc.contributor.authorDias, Ricardo
dc.contributor.authorOleastro, Mónica
dc.date.accessioned2018-02-20T16:11:31Z
dc.date.available2018-02-20T16:11:31Z
dc.date.issued2017-08
dc.description.abstractBackground: Helicobacter pylori virulence is associated with different clinical outcomes. The existence of an intact dupA gene from tfs4b cluster has been suggested as a predictor for duodenal ulcer development. However, the role of tfs plasticity zone clusters in the development of ulcers remains unclear. We studied several H. pylori strains to characterize the gene arrangement of tfs3 and tfs4 clusters and their impact in the inflammatory response by infected gastric cells. Methods: The genome of 14 H. pylori strains isolated from Western patients, pediatric (n=10) and adult (n=4), was fully sequenced using the Illumina platform MiSeq, in addition to eight pediatric strains previously sequenced. These strains were used to infect human gastric cells, and the secreted interleukin-8 (IL-8) was quantified by ELISA. The expression of virB2, dupA, virB8, virB10, and virB6 was assessed by quantitative PCR in adherent and nonadherent fractions of H. pylori during in vitro co-infection, at different pH values. Results: We have found that cagA-positive H. pylori strains harboring a complete tfs plasticity zone cluster significantly induce increased production of IL-8 from gastric cells. We have also found that the region spanning from virB2 to virB10 genes constitutes an operon, whose expression is increased in the adherent fraction of bacteria during infection, as well as in both adherent and nonadherent fractions at acidic conditions. Conclusions: A complete tfs plasticity zone cluster is a virulence factor that may be important for the colonization of H. pylori and to the development of severe outcomes of the infection with cagA-positive strains.pt_PT
dc.description.sponsorshipFundação para a Ciência e a Tecnologia, Grant/Award Number: FCT-PTDC/BIM-MEC/1051/2012 and SFRH/BPD/95125/2013.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationHelicobacter. 2017 Aug;22(4). doi: 10.1111/hel.12390. Epub 2017 Apr 24.pt_PT
dc.identifier.doi10.1111/hel.12390pt_PT
dc.identifier.issn1083-4389
dc.identifier.urihttp://hdl.handle.net/10400.18/5000
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherJohn Wiley & Sons Ltd.pt_PT
dc.relationMicro-lysis: studing Helicobacter pylori phages for phage therapy
dc.relation.publisherversionhttp://dx.doi.org/10.1111/hel.12390pt_PT
dc.subjectHelicobater pyloript_PT
dc.subjectAdhesionpt_PT
dc.subjectComplete tfs Clusterpt_PT
dc.subjectinterleukin-8pt_PT
dc.subjectOperonpt_PT
dc.subjectpHpt_PT
dc.subjectInfecções Gastrointestinaispt_PT
dc.titleThe expression of Helicobacter pylori tfs plasticity zone cluster is regulated by pH and adherence, and its composition is associated with differential gastric IL-8 secretionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMicro-lysis: studing Helicobacter pylori phages for phage therapy
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIM-MEC%2F1051%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F95125%2F2013/PT
oaire.citation.issue4pt_PT
oaire.citation.startPagee12390pt_PT
oaire.citation.titleHelicobacterpt_PT
oaire.citation.volume22pt_PT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication46fab5cc-442f-4ef1-a9e3-1ea2ec450999
relation.isProjectOfPublication6995404a-b07b-4c03-a272-7af723a52283
relation.isProjectOfPublication.latestForDiscovery46fab5cc-442f-4ef1-a9e3-1ea2ec450999

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