The 2020s tooth fairy: from loose tooth to neuronal cell cultures, a method to establish patient-derived MPS III neuronal cell lines

Coutinho, Maria Francisca; Santos, Juliana Inês; Matos, Liliana; Alves, Sandra

http://hdl.handle.net/10400.18/7461

Use this identifier to reference this record.

Name:	Description:	Size:	Format:
PO-11 SPDM2020 (pdf).pdf		1.91 MB	Adobe PDF	Download

Send Feedback

Authors

Coutinho, Maria Francisca

Santos, Juliana Inês

Matos, Liliana

Alves, Sandra

Abstract(s)

Here we present an alternative approach to generate patient-derived MPS III neuronal cell lines through a non-invasive, cost effective approach using dental pulp stem cells (DPSC). The plasticity of DPSCs has been demonstrated by several independent teams, who have shown that they self-maintain through several passages, holding potential to give rise to a variety of cells including functionally active neurons. Nevertheless, to the best of our knowledge, this sort of technology hasn’t yet been applied to samples obtained from LSD patients. Our goal is to establish one such protocol in house, to generate MPS III neurons from normally shed deciduous “baby” teeth. Once established, those neuronal cell lines will constitute a valuable platform to address further address RNA drugs’ efficiency in vitro.

Keywords

Lysosomal Storage Diseases MPS III Therapeutic RNA Drugs Dental Pulp Stem Cells Patient-derived MPS III Neuronal Cell Lines Doenças Genéticas Terapias de RNA