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Hygiene Indicators and Salmonellae on Surfaces of Swine Carcasses from Two Slaughterhouses in Northern Portugal

2022-11-01Journal article Restricted access
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dc.contributor.authorMoura-Alves, Márcio
dc.contributor.authorCarvalho, Marta
dc.contributor.authorBaggio Ribeiro, Deise Helena
dc.contributor.authorBarbosa, Joana
dc.contributor.authorSilveira, Leonor
dc.contributor.authorPista, Ângela
dc.contributor.authorPinto, Helena Patrícia
dc.contributor.authorSaraiva, Cristina
dc.contributor.authorTeixeira, Paula
dc.contributor.authorEsteves, Alexandra
dc.date.accessioned2023-03-21T15:34:59Z
dc.date.available2023-03-21T15:34:59Z
dc.date.issued2022-11-01
dc.description.abstractAbstract: The monitoring of carcass surface contamination along the slaughter line enables verification of slaughter operation hygiene and the use of good manufacturing practices. Pork meat is a common source of human nontyphoidal salmonellosis, one of the most frequently reported foodborne illnesses worldwide. This study was conducted to gather data on microbial loads before and after evisceration on the surfaces of swine carcasses in two slaughterhouses. The presence of Salmonella enterica was evaluated only after evisceration on carcass surfaces and in livers and floor drains (environmental samples) because pigs are common carriers of this pathogen. The contamination of carcass surfaces was evaluated by delimitation of surface area with sterilized templates (100 cm2), and surface samples were collected with gauze swabs. Total aerobic mesophilic bacteria, Enterobacteriaceae, and Escherichia coli were enumerated. Significant differences (P < 0.05) in counts of mesophilic bacteria, Enterobacteriaceae, and E. coli were found on the external carcass surfaces, with higher counts after evisceration. The neck and abdominal areas had higher levels of mesophilic bacteria, Enterobacteriaceae, and E. coli and a high prevalence of Salmonella. Salmonella was detected in only one of the studied slaughterhouses; 19 (7.3%) of 259 analyzed carcass samples were positive for Salmonella, and Salmonella was detected in two livers and two floor drains. A total of 52 Salmonella isolates (44 from carcasses, 5 from livers, and 3 from drains) were recovered. Three Salmonella serovars (Typhimurium 4,5:i- , Wernigerone, and Derby) were identified, and 53.8% of the 52 isolates were multidrug resistant. The results reveal the need for continuous improvement of slaughtering operations and implementation of good manufacturing practices to ensure the safety of pork produced in Portugal.pt_PT
dc.description.abstractHighlights: Higher counts of indicator microorganisms were obtained after swine evisceration; Salmonella was detected in 7.3% of swine carcass surface samples; Three Salmonella serovars were identified: Typhimurium 4,5:i− , Wernigerone, and Derby; Of the 52 Salmonella isolates, 53.8% were multidrug resistant.pt_PT
dc.description.sponsorshipThis work was funded by project UIDB/CVT/00772/2020 supported by the Fundação para a Ciência e Tecnologia (FCT). The authors are grateful for scientific collaboration under FCT project UID/Multi/50016/2019 and the financial support for J. Barbosa from a postdoctoral fellowship from FCT (SFRH/BPD/113303/2015)pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJ Food Prot. 2022 Nov 1;85(11):1566-1575. doi: 10.4315/JFP-21-312pt_PT
dc.identifier.doi10.4315/JFP-21-312pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/8592
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier/ International Association for Food Protectionpt_PT
dc.relationVeterinary and Animal Science Research Centre
dc.relationCentre for Biotechnology and Fine Chemistry
dc.relationClostridium difficile: prevalence in foods and study of alternative treatments to conventional therapeutics applied on C. difficile infection
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0362028X22110288?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectSalmonellapt_PT
dc.subjectAntimicrobial Susceptibilitypt_PT
dc.subjectContaminationpt_PT
dc.subjectPulsed-field Gel Electrophoresis Profilespt_PT
dc.subjectSlaughter Operationspt_PT
dc.subjectSwine Carcasspt_PT
dc.subjectInfecções Gastrointestinaispt_PT
dc.subjectPortugalpt_PT
dc.titleHygiene Indicators and Salmonellae on Surfaces of Swine Carcasses from Two Slaughterhouses in Northern Portugalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleVeterinary and Animal Science Research Centre
oaire.awardTitleCentre for Biotechnology and Fine Chemistry
oaire.awardTitleClostridium difficile: prevalence in foods and study of alternative treatments to conventional therapeutics applied on C. difficile infection
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00772%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F50016%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F113303%2F2015/PT
oaire.citation.endPage1575pt_PT
oaire.citation.issue11pt_PT
oaire.citation.startPage1566pt_PT
oaire.citation.titleJournal of Food Protectionpt_PT
oaire.citation.volume85pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicatione683cb7c-d293-4738-83c6-c3c95d798e4d
relation.isProjectOfPublication62ad761e-6507-4e6d-9064-a3d3fb9c57ad
relation.isProjectOfPublication2fc319ad-c2cd-48ff-afc1-a6228fa05c57
relation.isProjectOfPublication.latestForDiscoverye683cb7c-d293-4738-83c6-c3c95d798e4d

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