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Mutational Analysis of Colistin-Resistant Pseudomonas aeruginosa Isolates: From Genomic Background to Antibiotic Resistance

2025-04-15Artigo científico Acesso aberto
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dc.contributor.authorDe Sousa, Telma
dc.contributor.authorWang, Hsin-Yao
dc.contributor.authorLin, Ting-Wei
dc.contributor.authorCaniça, Manuela
dc.contributor.authorRamos, Miguel J.N.
dc.contributor.authorSantos, Daniela
dc.contributor.authorSilva, Catarina
dc.contributor.authorSaraiva, Sónia
dc.contributor.authorBeyrouthy, Racha
dc.contributor.authorBonnet, Richard
dc.contributor.authorHébraud, Michel
dc.contributor.authorIgrejas, Gilberto
dc.contributor.authorPoeta, Patrícia
dc.date.accessioned2026-02-06T13:44:21Z
dc.date.available2026-02-06T13:44:21Z
dc.date.issued2025-04-15
dc.description(This article belongs to the Special Issue Bacterial Resistance and Novel Therapeutic Approaches)
dc.description.abstractThis study analyzed eleven isolates of colistin-resistant Pseudomonas aeruginosa, originating from Portugal and Taiwan, which are associated with various pathologies. The results revealed significant genetic diversity among the isolates, with each exhibiting a distinct genetic profile. A prevalence of sequence type ST235 was observed, characterizing it as a high-risk clone, and serotyping indicated a predominance of type O11, associated with chronic respiratory infections in cystic fibrosis (CF) patients. The phylogenetic analysis demonstrated genetic diversity among the isolates, with distinct clades and complex evolutionary relationships. Additionally, transposable elements such as Tn3 and IS6 were identified in all isolates, highlighting their importance in the mobility of antibiotic resistance genes. An analysis of antimicrobial resistance profiles revealed pan-drug resistance in all isolates, with a high prevalence of genes conferring resistance to β-lactams and aminoglycosides. Furthermore, additional analyses revealed mutations in regulatory networks and specific loci previously implicated in colistin resistance, such as pmrA, cprS, phoO, and others, suggesting a possible contribution to the observed resistant phenotype. This study has a strong impact because it not only reveals the genetic diversity and resistance mechanisms in P. aeruginosa but also identifies mutations in regulatory genes associated with colistin resistance.eng
dc.description.sponsorshipThis research was funded by FCT (Fundação para a Ciência e a Tecnologia), related to a Ph.D. grant, with the reference 2020.05332.BD, and the projects and LA/P/0059/2020.
dc.identifier.citationPathogens. 2025 Apr 15;14(4):387. doi: 10.3390/pathogens14040387.
dc.identifier.doi10.3390/pathogens14040387
dc.identifier.eissn2076-0817
dc.identifier.pmid40333140
dc.identifier.urihttp://hdl.handle.net/10400.18/10825
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationEpidemiology of colistin-resistant Pseudomonas aeruginosa from humans, animals and ecosystems: a one health approach using omics tools OMIC4COLPA
dc.relationAssociate Laboratory for Animal and Veterinary Sciences
dc.relation.hasversionhttps://www.mdpi.com/2076-0817/14/4/387
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectPseudomonas aeruginosa
dc.subjectWGS
dc.subjectAntibiotic Resistance
dc.subjectColistin Resistance
dc.subjectMutations
dc.subjectResistência aos Antimicrobianos
dc.titleMutational Analysis of Colistin-Resistant Pseudomonas aeruginosa Isolates: From Genomic Background to Antibiotic Resistancepor
dc.typejournal article
dcterms.referenceshttps://www.mdpi.com/article/10.3390/pathogens14040387/s1
dspace.entity.typePublication
oaire.awardTitleEpidemiology of colistin-resistant Pseudomonas aeruginosa from humans, animals and ecosystems: a one health approach using omics tools OMIC4COLPA
oaire.awardTitleAssociate Laboratory for Animal and Veterinary Sciences
oaire.awardURIhttp://hdl.handle.net/10400.18/10810
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0059%2F2020/PT
oaire.citation.issue4
oaire.citation.startPage387
oaire.citation.titlePathogens
oaire.citation.volume14
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isProjectOfPublication74c5cbf6-71c5-423a-8907-2bd20a2e1bd2
relation.isProjectOfPublication93de5af7-dedc-4696-ae11-592584b020a8
relation.isProjectOfPublication.latestForDiscovery74c5cbf6-71c5-423a-8907-2bd20a2e1bd2

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