Evaluating mcyA gene expression in two toxic cyanobacterial species under different light intensities using RT-qPCR

Salvador, Daniel; Churro, Catarina; Valério, Elisabete

http://hdl.handle.net/10400.18/5651

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Authors

Salvador, Daniel

Churro, Catarina

Valério, Elisabete

Abstract(s)

The aim of this study was assess the influence of light intensity, in the transcription of the mcyA gene and corresponding production of microcystins in Microcystis aeruginosa and Planktothrix agardhii. For that purpose, cultures were exposed to three different light intensities (4, 20 and 30 μmol photons m−2 s−1) for 18 days at 20 ± 1ºC. The growth was followed daily using absorbance readings. At each growth stage samples were collected for cell counting, microcystins quantification by ELISA and RNA extraction. The level of transcripts was quantified by Real-Time RT-qPCR and the relative expression determined using three reference genes, 16S rRNA, gltA and rpoc1.