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Evaluating mcyA gene expression in two toxic cyanobacterial species under different light intensities using RT-qPCR
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Orientador(es)
Resumo(s)
The aim of this study was assess the influence of light intensity, in the transcription of the
mcyA gene and corresponding production of microcystins in Microcystis aeruginosa and
Planktothrix agardhii. For that purpose, cultures were exposed to three different light
intensities (4, 20 and 30 μmol photons m−2 s−1) for 18 days at 20 ± 1ºC. The growth was
followed daily using absorbance readings. At each growth stage samples were collected for cell
counting, microcystins quantification by ELISA and RNA extraction. The level of transcripts was
quantified by Real-Time RT-qPCR and the relative expression determined using three reference
genes, 16S rRNA, gltA and rpoc1.
Descrição
Palavras-chave
mcyA Gene Expression Microcystins Agentes Microbianos e Ambiente Água e Solo