Publication
Serotypes and Antibiotic Susceptibility of Streptococcus pneumoniae Isolates from Invasive Pneumococcal Disease and Asymptomatic Carriage in a Pre-vaccination Period, in Algeria
| dc.contributor.author | Ziane, Hanifa | |
| dc.contributor.author | Manageiro, Vera | |
| dc.contributor.author | Ferreira, Eugénia | |
| dc.contributor.author | Moura, Inês B. | |
| dc.contributor.author | Bektache, Soumia | |
| dc.contributor.author | Tazir, Mohamed | |
| dc.contributor.author | Caniça, Manuela | |
| dc.date.accessioned | 2017-02-17T11:27:04Z | |
| dc.date.available | 2017-02-17T11:27:04Z | |
| dc.date.issued | 2016-06-14 | |
| dc.description.abstract | In Algeria, few data is available concerning the distribution of pneumococcal serotypes and respective antibiotic resistance for the current pre-vaccination period, which is a public health concern. We identified the most frequent Streptococcus pneumoniae serogroup/types implicated in invasive pneumococcal disease (IPD; n = 80) and carriage (n = 138) in Algerian children younger than 5 years old. Serogroup/types of 78 IPD isolates were identified by capsular typing using a sequential multiplex PCR. Overall, serotypes 14, 19F, 6B, 23F, 18C, 1, 5, 7F, 19A, and 3 (55% of PCV7 serotypes, 71.3% of PCV10, and 90% of PCV13) were identified. Additionally, 7.5% of the non-vaccine serotypes 6C, 9N/L, 20, 24F, 35B, and 35F, were observed. In the case of S. pneumoniae asymptomatic children carriers, the most common serogroup/types were 6B, 14, 19F, 23F, 4, 9V/A, 1, 19A, 6A, and 3 (42.7% of PCV7 serotypes, 44.2% of PCV10, and 58% of PCV13). For 6.1% of the cases co-colonization was detected. Serotypes 14, 1, 5, and 19A were more implicated in IPD (p < 0.01), whereas serotype 6A was exclusively isolated from carriers (p < 0.01). Deaths associated with IPD were related to serotypes 19A, 14, 18C, and one non-typeable isolate. Among IPD related to vaccine serotypes, the rates of penicillin non-susceptible isolates were higher in no meningitis cases (80%) than in meningitis (66.7%), with serotypes 14, 19A, 19F, and 23F presenting the highest MIC levels (>2μg/ml). Resistance to cefotaxime was higher in isolates from meningitis (40.5%); however, resistance to erythromycin and co-trimoxazole (>40%) was more pronounced in no-meningeal forms. Overall, our results showed that PCV13 conjugate vaccine would cover up to 90% of the circulating isolates associated with IPD in Algeria, highlighting the importance of monitoring the frequency of S. pneumoniae serogroups/types during pre- and post-vaccination periods. | pt_PT |
| dc.description.sponsorship | HZ was supported by a grant from Ministère de l’Enseignement Superieur et de la Recherche Scientifique in Algeria. VM was supported by FCT fellowship (grant SFRH/BPD/77486/2011), financed by the European Social Funds (COMPETE-FEDER), and national funds of the Portuguese Ministry of Education and Science (POPH-QREN). | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Front Microbiol. 2016 Jun 14;7:803. doi: 10.3389/fmicb.2016.00803. eCollection 2016 | pt_PT |
| dc.identifier.doi | 10.3389/fmicb.2016.00803 | pt_PT |
| dc.identifier.issn | 1664-302X | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/4245 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Frontiers Media | pt_PT |
| dc.relation.publisherversion | http://journal.frontiersin.org/article/10.3389/fmicb.2016.00803/full | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | pt_PT |
| dc.subject | Streptococcus pneumoniae | pt_PT |
| dc.subject | Serotypes | pt_PT |
| dc.subject | Asymptomatic Children | pt_PT |
| dc.subject | Vaccine | pt_PT |
| dc.subject | Antibiotic Susceptibility | pt_PT |
| dc.subject | Algeria | pt_PT |
| dc.subject | Children | pt_PT |
| dc.subject | Invasive Disease | pt_PT |
| dc.subject | Pneumococcal Carriage | pt_PT |
| dc.subject | Pneumococci Serotype | pt_PT |
| dc.subject | Resistência aos Antimicrobianos | pt_PT |
| dc.title | Serotypes and Antibiotic Susceptibility of Streptococcus pneumoniae Isolates from Invasive Pneumococcal Disease and Asymptomatic Carriage in a Pre-vaccination Period, in Algeria | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.startPage | 803 | pt_PT |
| oaire.citation.title | Frontiers in Microbiology | pt_PT |
| oaire.citation.volume | 7 | pt_PT |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |