Publication
Impact of farnesol and Corsodyl® on Candida albicans forming dual biofilm with Streptococcus mutans
| dc.contributor.author | Černáková, Lucia | |
| dc.contributor.author | Jordao, Luisa | |
| dc.contributor.author | Bujdáková, Helena | |
| dc.date.accessioned | 2018-04-24T15:40:57Z | |
| dc.date.available | 2020-04-01T00:30:14Z | |
| dc.date.issued | 2018-04-18 | |
| dc.description.abstract | Objective: This work studied the biofilm formed by Candida albicans and Streptococcus mutans on a hydroxyapatite surface after exposure to the quorum-sensing molecule farnesol (200 µM) in comparison with the diluted mouthwash Corsodyl® (0.0001% chlorhexidine digluconate). Materials and Methods: The cytotoxicity of farnesol was evaluated by Galleria melonella surviving assay. The viability of biofilm cells after exposure to farnesol and Corsodyl® was determined by colony-forming units. The morphology and structure of a dual-species biofilm was evaluated by scanning electron microscopy. Results: Farnesol did not exhibit a toxic effect on larval survival. While 200 µM farnesol effectively reduced the yeast-to-hyphae transition in the dual biofilm, it did not affect the growth of S. mutans. Additionally, despite the presence of farnesol, many blastospores were observed. Corsodyl® reduced S. mutans in the dual biofilm, but did not influence C. albicans. Conclusion: This study showed that 200 µM farnesol modulated C. albicans in a dual-species biofilm with S. mutans, but did not exhibit antimicrobial activity against S. mutans. Moreover, it seems that S. mutans provides conditions that support the growth of the yeast form of C. albicans. The mouthwash Corsodyl® reduces S. mutans, but was not effective against C. albicans. | pt_PT |
| dc.description.sponsorship | We would like to thank COST Action TD1305 Improved Protection of Medical Devices against Infection (iPROMEDAI) for funding the Short-term scientific mission at the Instituto Nacional de Saúde Dr Ricardo Jorge, Departamento de Saúde Ambiental, Unidade de Investigação e Desenvolvimento-Lisboa, Avenida Padre Cruz, Lisboa, Portugal. This research was also funded by the Slovak Research and Development Agency under the contract No. [APVV-15-0347] and by the grant VEGA [1/0628/15] supported by the Ministry of Education, Science, Research and Sport of the Slovak Republic. We wish to thank to Isabel Nogueira from MicroLab–Instituto Superior Técnico in Lisbon, for her expert assistance with scanning electron microscopy. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Oral Dis. 2018 Sep;24(6):1126-1131. doi: 10.1111/odi.12873. Epub 2018 Apr 18. | pt_PT |
| dc.identifier.doi | 10.1111/odi.12873 | pt_PT |
| dc.identifier.issn | 1354-523X | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/5541 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Wiley/European Association of Oral Medicine | pt_PT |
| dc.relation.publisherversion | http://dx.doi.org/10.1111/odi.12873 | pt_PT |
| dc.subject | Candida Albicans | pt_PT |
| dc.subject | Streptococcus Mutans | pt_PT |
| dc.subject | Dental Biofilm | pt_PT |
| dc.subject | Quorum Sensing | pt_PT |
| dc.subject | Hydroxyapatite | pt_PT |
| dc.subject | Morphology | pt_PT |
| dc.title | Impact of farnesol and Corsodyl® on Candida albicans forming dual biofilm with Streptococcus mutans | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.title | Oral Diseases | pt_PT |
| rcaap.embargofct | De acordo com a politica da revista | pt_PT |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |