Human mTOR mRNA allows cap-independent translation that insures its expression and function during inhibition of global translation

Marques-Ramos, Ana; Candeias, Marco; Menezes, Juliane; Lacerda, Rafaela; Willcocks, M.; Locker, Nicolas; Romão, Luísa

Publication

Human mTOR mRNA allows cap-independent translation that insures its expression and function during inhibition of global translation

2017-05-30Conference object

dc.contributor.author	Marques-Ramos, Ana
dc.contributor.author	Candeias, Marco
dc.contributor.author	Menezes, Juliane
dc.contributor.author	Lacerda, Rafaela
dc.contributor.author	Willcocks, M.
dc.contributor.author	Locker, Nicolas
dc.contributor.author	Romão, Luísa
dc.date.accessioned	2018-03-02T16:27:34Z
dc.date.available	2018-03-02T16:27:34Z
dc.date.issued	2017-05-30
dc.description.abstract	The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that integrates signals from the cellular nutrient- and energy-status, acting namely on the protein synthesis machinery. Deregulation of mTOR signaling is implicated in major diseases, such as cancer, mainly due to its role in regulating protein synthesis. Major advances are emerging regarding the regulators and effects of mTOR signaling pathway; however, regulation of mTOR gene expression is not well known. Here, we show that the 5’ untranslated region of the human mTOR transcript forms a highly folded RNA scaffold capable of binding directly to the 40S ribosomal subunit. We further demonstrate that this cis-acting RNA regulon is active both in normal and stress conditions, and that its activation status in response to translational adverse conditions parallels mTOR protein levels. Moreover, our data reveal that the cap-independent translation of mTOR is necessary for its ability to induce cell cycle progression into S-phase. These results suggest a novel regulatory mechanism of mTOR gene expression that integrates the protein profile rearrangement triggered by global translation inhibitory conditions.	pt_PT
dc.description.version	N/A	pt_PT
dc.identifier.uri	http://hdl.handle.net/10400.18/5136
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by-nc/4.0/	pt_PT
dc.subject	Mammalian Target of Rapamycin (mTOR)	pt_PT
dc.subject	Expressão Génica	pt_PT
dc.subject	Genómica Funcional e Estrutural	pt_PT
dc.title	Human mTOR mRNA allows cap-independent translation that insures its expression and function during inhibition of global translation	pt_PT
dc.type	conference object
dspace.entity.type	Publication
oaire.citation.conferencePlace	Prague, Czech Republic	pt_PT
oaire.citation.title	22nd Annual Meeting of the RNA Society, 30 May -3 June 2017	pt_PT
rcaap.rights	openAccess	pt_PT
rcaap.type	conferenceObject	pt_PT