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Antimicrobial Resistance and Biofilms Underlying Catheter-Related Bloodstream Coinfection by Enterobacter cloacae Complex and Candida parapsilosis

2022-09-14Journal article Open access
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dc.contributor.authorŠtefánek, Matúš
dc.contributor.authorWenner, Sigurd
dc.contributor.authorBorges, Vítor
dc.contributor.authorPinto, Miguel
dc.contributor.authorGomes, João Paulo
dc.contributor.authorRodrigues, João
dc.contributor.authorFaria, Isabel
dc.contributor.authorPessanha, Maria Ana
dc.contributor.authorMartins, Filomena
dc.contributor.authorSabino, Raquel
dc.contributor.authorVeríssimo, Cristina
dc.contributor.authorNogueira, Isabel D.
dc.contributor.authorCarvalho, Patrícia Almeida
dc.contributor.authorBujdáková, Helena
dc.contributor.authorJordao, Luisa
dc.date.accessioned2022-11-15T12:31:51Z
dc.date.available2022-11-15T12:31:51Z
dc.date.issued2022-09-14
dc.descriptionThis article belongs to the Special Issue Microbial Biofilms, Antimicrobials, and Virulence Determinants.pt_PT
dc.description.abstractBiofilm-associated infections are a public health concern especially in the context of healthcare-associated infections such as catheter-related bloodstream infections (CRBSIs). We evaluated the biofilm formation and antimicrobials resistance (AMR) of Enterobacter cloacae complex and Candida parapsilosis co-isolated from a CRBSI patient. Antimicrobial susceptibility of central venous catheters (CVCs) and hemoculture (HC) isolates was evaluated, including whole genome sequencing (WGS) resistome analysis and evaluation of gene expression to obtain insight into their AMR determinants. Crystal violet assay was used to assess dual biofilm biomass and microscopy was used to elucidate a microorganism’s distribution within biofilms assembled on different materials. Bacteria were multidrug-resistant including resistance to colistin and beta-lactams, likely linked to the mcr-9-like phosphoethanolamine transferase and to an ACT family cephalosporin-hydrolyzing class C beta-lactamase, respectively. The R398I and Y132F mutations in the ERG11 gene and its differential expression might account for C. parapsilosis resistance to fluconazole. The phenotype of dual biofilms assembled on glass, polystyrene and polyurethane depends on the material and how biofilms were initiated by one or both pathogens. Biofilms assembled on polyurethane were denser and richer in the extracellular polymeric matrix, and microorganisms were differently distributed on the inner/outer surface of the CVC.pt_PT
dc.description.sponsorshipThis research was funded by Portuguese Fundação para a Ciência e a Tecnologia and by the Slovak Research and Development Agency under contracts of SK-PT-18-0006 as a part of the Bilateral Cooperation Program (2019–2022) and APVV-21-0302, and by the grant VEGA 1/0537/19 supported by the Ministry of Education, Science, Research and Sport of the Slovak Republic.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAntibiotics (Basel). 2022 Sep 14;11(9):1245. doi: 10.3390/antibiotics11091245pt_PT
dc.identifier.doi10.3390/antibiotics11091245pt_PT
dc.identifier.issn2079-6382
dc.identifier.urihttp://hdl.handle.net/10400.18/8311
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2079-6382/11/9/1245pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBiofilmspt_PT
dc.subjectCatheter-related Bloodstream Infectionspt_PT
dc.subjectPolymicrobial Biofilmspt_PT
dc.subjectAntimicrobial Resistancept_PT
dc.subjectMicroscopypt_PT
dc.subjectWhole Genome Sequencingpt_PT
dc.subjectResistência aos Antimicrobianospt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.titleAntimicrobial Resistance and Biofilms Underlying Catheter-Related Bloodstream Coinfection by Enterobacter cloacae Complex and Candida parapsilosispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue9pt_PT
oaire.citation.startPage1245pt_PT
oaire.citation.titleAntibioticspt_PT
oaire.citation.volume11pt_PT
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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