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Brazil nut prevents oxidative DNA damage in type 2 diabetes patients

2020-08-18Journal article Restricted access
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dc.contributor.authorMacan, Tamires Pavei
dc.contributor.authorde Amorim, Thais Aquino
dc.contributor.authorDamiani, Adriani Paganini
dc.contributor.authorBeretta, Ângela Caroline da Luz
dc.contributor.authorMagenis, Marina Lummertz
dc.contributor.authorVilela, Thais Ceresér
dc.contributor.authorTeixeira, João Paulo
dc.contributor.authorAndrade, Vanessa Moraes de
dc.date.accessioned2021-03-03T18:14:38Z
dc.date.available2021-03-03T18:14:38Z
dc.date.issued2020-08-18
dc.description.abstractThe Brazil nut (Bertholletia excelsa, H.B.K.) originating from the Amazon region is one of the richest known sources of selenium (Se), a micronutrient that is essential and required for optimal physiological functioning. This mineral presents several health benefits, including improvement of the redox cellular status and maintenance of genomic stability. Knowing that type 2 diabetes mellitus (T2D) is strongly linked to oxidative stress and consequently DNA damage, the aim of this study was to assess the ex vivo antioxidative effects of Se through Brazil nut consumption and its potential in preventing oxidative DNA damage induced by H2O2. In order to accomplish this, the Comet assay (single-cell gel electrophoresis) was used to measure DNA damage in peripheral blood cells harvested before and after supplementation with Brazil nut. Comet assay was also applied ex vivo to measure the potential of Se to prevent oxidative damage to DNA induced by H2O2 in blood of type 2 diabetes patients collected before and after six months of supplementation with Brazil nut. We found that supplementation with Brazil nuts significantly increased serum Se levels. Furthermore, we observed a significant increase in fasting blood glucose after six months of consuming Brazil nuts; however, no significant effect was observed on the levels of glycated hemoglobin. Finally, we noticed that the cells were more resistant to H2O2-induced DNA damage after six months of supplementation with Brazil nut. Thus, consumption of Brazil nuts could decrease oxidative DNA damage in T2D patients, probably through the antioxidative effects of Se.pt_PT
dc.description.sponsorshipThis work was supported by the Santa Catarina State Research and Innovation Support Foundation (FAPESC, Brazil) under Grant number ACA2017031000023; Coordination for the Improvement of Higher Education Personnel (CAPES, Brazil) under Grant number 2794/2014; National Council of Technological and Scientific Development (CNPq, Brazil) under Grant number 407261/2016–8; and Graduate Program in Health Sciences (PPGCS) of the University of Southern Santa Catarina (UNESC, Brazil).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationDrug Chem Toxicol. 2022 May;45(3):1066-1072. doi: 10.1080/01480545.2020.1808667. Epub 2020 Aug 18pt_PT
dc.identifier.doi10.1080/01480545.2020.1808667pt_PT
dc.identifier.issn0148-0545
dc.identifier.urihttp://hdl.handle.net/10400.18/7300
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relation.publisherversionhttps://www.tandfonline.com/doi/abs/10.1080/01480545.2020.1808667?journalCode=idct20pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectBrazil Nutpt_PT
dc.subjectSeleniumpt_PT
dc.subjectAntioxidantpt_PT
dc.subjectHyperglycemiapt_PT
dc.subjectOverweightpt_PT
dc.subjectDNA Damagept_PT
dc.subjectComet Assaypt_PT
dc.subjectH2O2pt_PT
dc.subjectGenotoxicidade Ambiental
dc.titleBrazil nut prevents oxidative DNA damage in type 2 diabetes patientspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage7pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleDrug and Chemical Toxicologypt_PT
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

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