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Browsing by Author "Walder, Gernot"

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  • Practical Guidelines for Studies on Sandfly-Borne Phleboviruses: Part I: Important Points to Consider Ante Field Work
    Publication . Ayhan, Nazli; Baklouti, Amal; Prudhomme, Jorian; Walder, Gernot; Amaro, Fatima; Alten, Bulent; Moutailler, Sara; Ergunay, Koray; Charrel, Remi N; Huemer, Hartwig
    The purpose of this review is to provide practical information to help researchers intending to perform "from field to laboratory" studies on phleboviruses transmitted by sandflies. This guideline addresses the different steps to be considered starting from the field collection of sandflies to the laboratory techniques aiming at the detection, isolation, and characterization of sandfly-borne phleboviruses. In this guideline article, we address the impact of various types of data for an optimal organization of the field work intending to collect wildlife sandflies for subsequent virology studies. Analysis of different data sets should result in the geographic positioning of the trapping stations. The overall planning, the equipment and tools needed, the manpower to be deployed, and the logistics to be anticipated and set up should be organized according to the objectives of the field study for optimal efficiency.
    2017Journal article Restricted access Show more
  • Practical Guidelines for Studies on Sandfly-Borne Phleboviruses: Part II: Important Points to Consider for Fieldwork and Subsequent Virological Screening
    Publication . Huemer, Hartwig; Prudhomme, Jorian; Amaro, Fatima; Baklouti, Amal; Walder, Gernot; Alten, Bulent; Moutailler, Sara; Ergunay, Koray; Charrel, Remi N.; Ayhan, Nazli
    In this series of review articles entitled "Practical guidelines for studies on sandfly-borne phleboviruses," the important points to be considered at the prefieldwork stage were addressed in part I, including parameters to be taken into account to define the geographic area for sand fly trapping and how to organize field collections. Here in part II, the following points have been addressed: (1) factors influencing the efficacy of trapping and the different types of traps with their respective advantages and drawbacks, (2) how to process the trapped sand flies in the field, and (3) how to process the sand flies in the virology laboratory. These chapters provide the necessary information for adopting the most appropriate procedures depending on the requirements of the study. In addition, practical information gathered through years of experience of translational projects is included to help newcomers to fieldwork studies.
    2017-01Journal article Restricted access Show more