Percorrer por autor "Senok, Abiola"
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- Methicillin-Resistant Staphylococcus aureus: The Shifting Landscape in the United Arab EmiratesPublication . Boucherabine, Syrine; Nassar, Rania; Mohamed, Lobna; Habous, Maya; Nabi, Anju; Husain, Riyaz Amirali; Alfaresi, Mubarak; Oommen, Seema; Khansaheb, Hamda Hassan; Al Sharhan, Mouza; Celiloglu, Handan; Raja, Mubarak Hussain; Abdelkarim, Eman; Ali, Nishi; Tausif, Salman; Olowoyeye, Victory; Soares, Nelson Cruz; Hachim, Mahmood; Moradigaravand, Danesh; Everett, Dean; Mueller, Elke; Monecke, Stefan; Ehricht, Ralf; Senok, AbiolaBackground: Methicillin-resistant Staphylococcus aureus (MRSA) is a significant burden globally, particularly in the Arabian Gulf region. The United Arab Emirates (UAE) has experienced rising MRSA prevalence, with increasing diversity in the clonal complexes (CCs) identified. The COVID-19 pandemic, with its increased hospitalization rates and antibiotic use, may have further influenced MRSA's genetic evolution and epidemiology in the country. Methods: To investigate this influence, genomic profiling of 310 MRSA clinical isolates collected between February and November 2022 was performed using a DNA microarray-based assay. Results: Isolates were assigned to 22 clonal complexes and 72 distinct strain assignments. The predominant clonal complexes were CC5, CC6, CC361, CC22, CC1, and CC8. Community-acquired MRSA lineages were dominant, with only one healthcare-associated MRSA lineage isolate identified. Upward trends of CC1153 were observed along with rare CCs, such as CC121-MRSA and CC7-MRSA, with the latter being reported for the first time in the Arabian Gulf region. The presence of pandemic strains USA300 CC8-MRSA-[IVa + ACME1] and CC8-MRSA-IV strains were also observed, including variants lacking Panton-Valentine leukocidin (pvl) genes and missing tst1 or enterotoxin genes. The PVL-negative CC772-MRSA-V/VT was identified, representing its first report in the UAE. A novel variant, CC361-MRSA-IV (tst1+/PVL+), was identified. Pvl genes were observed in 36% of the isolates, primarily from skin and soft tissue infections, while fusC (SCC-borne fusidic acid resistance) was identified in 13% of the isolates. Conclusions: The findings highlight the ongoing evolution of MRSA in the UAE, with the persistence and emergence of diverse and rare clonal complexes, driving the need for continuous genomic surveillance.
- Proteomic and metabolomic profiling of methicillin-resistant Staphylococcus aureus associated with invasive vs. non-invasive infections: uncovering key biomarkers and pathogenic pathwaysPublication . Boucherabine, Syrine; Giddey, Alexander D.; Nassar, Rania; Mohamed, Lobna; Verma, Subham; Soares, Nelson C.; Senok, AbiolaIntroduction: Understanding the behavioral differences between invasive and non-invasive methicillin-resistant Staphylococcus aureus (MRSA) is essential for unraveling infection mechanisms and identifying biomarkers with translational potential. This study compared the proteomic and metabolomic profiles of MRSA isolates from diverse clinical presentations to uncover distinct molecular signatures. Methods: Invasive isolates were obtained from blood cultures (n = 23), while non-invasive isolates were derived from superficial skin infections (n = 49) and nasal colonizers (n = 24) from screening swabs. Proteins and metabolites were simultaneously extracted using a dual-phase methanol-based protocol. Proteomic analysis was performed on the Orbitrap Exploris 480, while metabolites were characterized using a TimsTOF mass spectrometer with an Apollo II electrospray ionization source. Data-independent acquisition (DIA) was applied, with peptide assignment carried out in DIA-NN and metabolite analysis using MetaboScape® 4.0. Results: Across all isolates, 2,000 proteins and 150 metabolites were identified. Comparative analysis revealed that invasive isolates exhibited consistently higher levels of two metabolites (sphinganine and phosphoserine) and one protein (staphylococcal secretory antigen SsaA2) compared to non-invasive. In contrast, three metabolites (cytidine, benzoic acid, and guanosine) and two proteins (small ribosomal subunit protein bS20 and bifunctional autolysin) were significantly reduced in invasive isolates. These findings highlight key molecular differences underpinning invasive potential in MRSA, providing insights into candidate diagnostic and therapeutic biomarkers. These findings highlight critical biological differences between invasive and non-invasive MRSA, offering valuable insights into potential diagnostic and therapeutic biomarkers.