Percorrer por autor "Rabilloud, Thierry"
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- The transition of the European Proteomics Association into the futurePublication . Corthals, Gerry L.; Dunn, Mike; James, Peter; Gil, Concha; Penque, Deborah; Albar, Juan Pablo; Andrén, Per; Rabilloud, Thierry; Marko-Varga, GyorgyThe following report provides an overview of the discussions and outcome of the EuPA General Council meeting that took place in Estoril 20–21 October 2010. During the annual meeting future policy and action plans in a variety of areas are decided. Several important points were decided upon during this meeting including the expansion of the EuPA Executive Committee by introducing a new EuPA committee – EuPA Developments – that will initially spearhead activities in standardisation, imaging ms and biobanking. The EuPA General Council also invited Russia as its 17th member. More details about these and additional activities are presented in the article.
- Toxicological impact of acute exposure to E171 food additive and TiO2 nanoparticles on a co-culture of Caco-2 and HT29-MTX intestinal cellsPublication . Dorier, Marie; Tisseyre, Céline; Dussert, Fanny; Béal, David; Arnal, Marie-Edith; Douki, Thierry; Valdiglesias, Vanessa; Laffon, Blanca; Fraga, Sónia; Brandão, Fátima; Herlin-Boime, Nathalie; Barreau, Frédérick; Rabilloud, Thierry; Carriere, MarieTiO2 particles are widely used in products for everyday consumption, such as cosmetics and food; their possible adverse effects on human health must therefore be investigated. The aim of this study was to document in vitro impact of the food additive E171, i.e. TiO2, and of TiO2 nanoparticles, on a co-culture of Caco-2 and HT29-MTX cells, which is an in vitro model for human intestine. Cells were exposed to TiO2 particles three days after seeding, i.e. while they were not fully differentiated. Cell viability, reactive oxygen species (ROS) levels and DNA integrity were assessed, by MTT assay, DCFH-DA assay, alkaline and Fpg-modified comet assay and 8-oxo-dGuo measurement by HPLC-MS/MS. The mRNA expression of genes involved in ROS regulation, DNA repair via base-excision repair, and endoplasmic reticulum stress was assessed by RT-qPCR. Exposure to TiO2 particles resulted in increased intracellular ROS levels, but did not impair cell viability and did not cause any oxidative damage to DNA. Only minor changes in mRNA expression were detected. Altogether, this shows that E171 food additive and TiO2 nanoparticles only produce minor effects to this in vitro intestinal cell model.