Browsing by Author "Pinto, Margarida"
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- Complete Sequence of a blaOXA-48-Harboring IncL Plasmid from an Enterobacter cloacae Clinical IsolatePublication . Manageiro, Vera; Pinto, Margarida; Caniça, ManuelaWe report a 63,584-bp conjugative IncL plasmid (pUR17313-1) from an Enterobacter cloacae clinical isolate, containing a blaOXA-48 gene. The plasmid sequence also carried important mobile genetic elements involved in the spread of antibiotic resistance, namely, the Tn1999.2 composite transposon, which enclosed blaOXA-48-, integrase-, and transposase-encoding genes.
- Emergence of β-lactamase-mediated resistance to oxyimino-β-lactams in Enterobacteriaceae isolates in various services in a single centrePublication . Manageiro, Vera; Ferreira, Eugénia; Jones-Dias, Daniela; Louro, Deolinda; Pinto, Margarida; Diogo, José; Caniça, ManuelaWe studied 193 Enterobacteriaceae isolates presenting diminished susceptibility to oxyimino-cephalosporins recovered in a Portuguese hospital (2004–2008). CTX-M-3 producers, firstly detected in Portugal, were associated with a Klebsiella pneumoniae microepidemic clone. Production of CTX-M–type enzymes (CTX-M-1/-3/-9/-14/-15/-32), age ≥65 years, and nosocomial infection were risk factors for higher nonsusceptibility to oxyimino-β-lactams. CMY-2 and DHA-1 β-lactamases were only identified in 1% of isolates.
- First description of OXA-48 carbapenemase harbored by Escherichia coli and Enterobacter cloacae from a single patient in PortugalPublication . Manageiro, Vera; Ferreira, Eugénia; Pinto, Margarida; Caniça, ManuelaThe blaOXA-48 gene has been detected in a variety of species and clones of Enterobacteriaceae, circulating mainly throughout the Mediterranean area but progressively disseminating to other geographical areas. To our knowledge, we describe here the first cases of OXA-48-producing Enterobacteriaceae in Portugal. Enterobacter cloacae and Escherichia coli were isolated from the urine of a catheterized patient.
- Sequenciação de Nova Geração no apoio à decisão de transplante pulmonar num doente com fibrose quísticaPublication . Silveira, Leonor; Casimiro, Ana; Pinto, Margarida; Borges, Vítor; Gomes, João Paulo; Oleastro, MónicaObjetivo: Neste estudo reportamos a aplicação desta tecnologia no contexto de apoio à decisão clínica de transplante pulmonar num doente com Fibrose Quística.
- Two novel CMY-2-type β-lactamases encountered in clinical Escherichia coli isolatesPublication . Manageiro, Vera; Ferreira, Eugénia; Pinto, Margarida; Fonseca, Fernando; Ferreira, Mónica; Bonnet, Richard; Caniça, ManuelaBACKGROUND: Chromosomally encoded AmpC β-lactamases may be acquired by transmissible plasmids which consequently can disseminate into bacteria lacking or poorly expressing a chromosomal bla AmpC gene. Nowadays, these plasmid-mediated AmpC β-lactamases are found in different bacterial species, namely Enterobacteriaceae, which typically do not express these types of β-lactamase such as Klebsiella spp. or Escherichia coli. This study was performed to characterize two E. coli isolates collected in two different Portuguese hospitals, both carrying a novel CMY-2-type β-lactamase-encoding gene. FINDINGS: Both isolates, INSRA1169 and INSRA3413, and their respective transformants, were non-susceptible to amoxicillin, amoxicillin plus clavulanic acid, cephalothin, cefoxitin, ceftazidime and cefotaxime, but susceptible to cefepime and imipenem, and presented evidence of synergy between cloxacilin and cefoxitin and/or ceftazidime. The genetic characterization of both isolates revealed the presence of bla CMY-46 and bla CMY-50 genes, respectively, and the following three resistance-encoding regions: a Citrobacter freundii chromosome-type structure encompassing a blc-sugE-bla CMY-2-type -ampR platform; a sul1-type class 1 integron with two antibiotic resistance gene cassettes (dfrA1 and aadA1); and a truncated mercury resistance operon. CONCLUSIONS: This study describes two new bla CMY-2-type genes in E. coli isolates, located within a C. freundii-derived fragment, which may suggest their mobilization through mobile genetic elements. The presence of the three different resistance regions in these isolates, with diverse genetic determinants of resistance and mobile elements, may further contribute to the emergence and spread of these genes, both at a chromosomal or/and plasmid level.