Browsing by Author "Oliveira, M.M."
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- Effects of Trihalomethanes on Liver MitochondriaPublication . Faustino-Rocha, A.I.; Rodrigues, D.; Gil da Costa, R.M.; Dinis, C.; Talhada, D.; Aragão, S.; Botelho, M.; Colaço, A; Pires, M.J.; Oliveira, M.M.; Peixoto, F; Oliveira, P.A.Introduction: Trihalomethanes (THMs), namely dibromochloromethane (DBCM) and bromodichloromethane (BDCM), are disinfection byproducts of chlorinated water. This experiment aimed to evaluate the mitochondrial dysfunction induced by THMs at low levels in a mouse model.
- In vitro culture may be the major contributing factor for transgenic versus nontransgenic proteomic plant differencesPublication . Fonseca, C.; Planchon, S.; Serra, T.; Chander, S.; Saibo, N.J.M.; Renaut, J.; Oliveira, M.M.; Batista, RitaIdentification of differences between genetically modified plants and their original counterparts plays a central role in risk assessment strategy. Our main goal was to better understand the relevance of transgene presence, genetic, and epigenetic changes induced by transgene insertion, and in vitro culture in putative unintended differences between a transgenic and its comparator. Thus, we have used multiplex fluorescence 2DE coupled with MS to characterize the proteome of three different rice lines (Oryza sativa L. ssp. japonica cv. Nipponbare): a control conventional line (C), an Agrobacterium-transformed transgenic line (Ta) and a negative segregant (NSb). We observed that Ta and NSb appeared identical (with only one spot differentially abundant--fold difference ≥ 1.5), contrasting with the control (49 spots with fold difference ≥ 1.5, in both Ta and NSb vs. control). Given that in vitro culture was the only event in common between Ta and NSb, we hypothesize that in vitro culture stress was the most relevant condition contributing for the observed proteomic differences. MS protein identification support our hypothesis, indicating that Ta and NSb lines adjusted their metabolic pathways and altered the abundance of several stress related proteins in order to cope with in vitro culture.
- Safer to eat transgenic than environmentally stressed plants?Publication . Batista, Rita; Planchon, S.; Fonseca, C.; Negrão, S.; Renaut, J.; Oliveira, M.M.Concerns about Biosafety of transgenic crops have led Governments to implement regulations to assess potential risks before genetically engineered crops are approved for commercialization. Each genetically modified crop regulatory approval is preceded by years of intensive research demonstrating that the crop is safe to humans, animals as well as other non-target beneficial organisms, plants and environment. In a recent study, we have demonstrated that the major factor influencing proteomic differences of transgenic vs. non-transgenic plants may be the in vitro culture stress imposed during plant genetic transformation. This study lead us to question the dimension of transgenic vs. non transgenic differences as compared with the ones we know that can happen any time, due to environmental stresses.