Browsing by Author "Mauro, Raul"
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- Protein and Amino Acids Profile of Andean Cereals from North of ArgentinaPublication . Mota, Carla; Mauro, Raul; Samman, Norma; Santos, Mariana; Castanheira, IsabelQuinoa (Chenopodium Quinoa) amaranth (Amaranthus caudatus) and corn purple (Zea mays L.) are crops with large genetic variability adapted to diverse habitats agro-climatic and edaphic conditions. Few Food Composition Databanks include data on their amino acid profile (AAP). The aim of work was characterized AAP of Quinoa, Amaranth and Corn purple consumed in the North of Argentina and originated from Jujuy Province and Salta and compare with rice as the most consumed gluten free cereal in Europe. Study was framed by EuroFIR quality criteria to guarantee comparability of results that enter in Argentine, LATINFOODS Food Composition Data and EuroFIR e-search platform. Samples in triplicate were collected from local market of Salta and Jujuy. Rice was collected from most representative Portuguese rice producers. Protein quantification was carried out by Kjeldahl method (AOAC920.87). Amino acids (AA) were analyzed by UPLC system equipped with diode array detector (260 nm), using pre-column derivatization preceded by acid hydrolysis (6N HCl) in microwave system. The method was used for determination of aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, valine, isoleucine, leucine, tyrosine, phenylalanine, histidine, lysine, arginine, methionine and cysteine. Total Protein content varies from 16.3 g/100g (quinoa) to 10.5 g/100g (purple corn) lower values were found in rice samples (6.7 g/100g). Most abundant essential AA was aromatic amino acids (AAA). Quinoa presents the highest content in AAA (153.5 mg/100g). Amaranth was found the cereal with a major methionine content (28.9 mg/100g). Purple corn has the minor content in lysine (6.4 mg/100g) and major in leucine (106.6 mg/100g). Rice presents the lowest content for all AA. The research showed that Andeans cereals are a better source of AA than rice. EuroFIR guidelines proved to be a crucial tool to guarantee the comparability of data.
- Protein and amino acids profile of andean cereals from North of ArgentinaPublication . Mota, Carla; Mauro, Raul; Samman, Norma; Santos, Mariana; Torres, Duarte; Castanheira, Isabel
- Protein content and amino acid profile in Portuguese ricePublication . Mota, Carla; Cabral, Margarida; Mauro, Raul; Torres, Duarte; Santos, Mariana; André, Catarina; Castanheira, IsabelAccording to INE, between 2010 and 2011, rice (Oryza sativa) availability in Portugal was 15.8 kg per capita per day, which represents the highest in Europe [1]. Rice amino acids profile evaluation is important to define the nutritional quality of different species contributing to choice of the varieties of rice to grow. This study aimed to quantify the total protein and amino acids profile in 15 cultivated accessions selected from the two major subspecies, Japonica and Indica, grown in Portuguese Sado and Ribatejo regions between 2009 and 2011. Total protein was determined by Kjeldahl method and the content of AA was measured by an Ultra Performance Liquid Chromatography of ACQUITY ™ UPLC ® (Waters) system equipped with a photodiode array detector (DAD) with a pre-derivatisation column 6-N-aminoquinolil hidroxisuccinimidil carbamate, after microwave acid hydrolysis. Analysis of the results emphasizes that there was no significant difference between Japonica varieties (6.4 ± 0.5 g/100g) and Indica (7.0 ± 1.6 g/100g) in relation to total protein content. Amino acids score, as defined by WHO/FAO/UNU [2], was between 0.55 (lysine) and 3.24 (aromatic amino acids). Lysine was the only limiting AA (score <1). No significant differences were observed in amino content between subspecies, cultivation region and year of crop. The obtained results may be aggregated and include in the Portuguese Food Composition Databank and EuroFIR Food Platform [3]. Research on wholegrain and husk rice properties is underway and will be discussed.
- Protein content and amino acids profile of pseudocerealsPublication . Motta, Carla; Santos, Mariana; Mauro, Raul; Matos, Ana; Torres, Duarte; Castanheira, IsabelQuinoa (Chenopodium quinoa), amaranth (Amaranthus caudatus) and buckwheat (Fagopyrum esculentum) represent the main protein source in several diets, although these pseudocereals are not currently present in the FCDB nutrient profile information. The aim of this work is to characterise the AA profile of these pseudocereals and compare them with rice. Total protein content revealed to vary from 16.3 g/100 g (quinoa Salta) to 13.1 g/100 g (buckwheat) and lower values were found in rice samples (6.7 g/100 g). For pseudocereals the most abundant essential AA was leucine. Quinoa-Salta evidences the highest leucine content (1013 mg/100 g) and the minor methionine content (199 mg/100 g). Buckwheat was the cereal with the highest phenylalanine content (862 mg/100 g). Rice (Oryza sativa) presents the lowest content for all AA. Results showed pseudocereals as the best source of AA. EuroFIR guidelines where strictly followed and proved to be a crucial tool to guarantee data interchangeability and comparability.